Abstract: PO0377
Hypertensive Diabetic Kidney Disease Increases the Severity of Experimental AKI
Session Information
- AKI: Mechanisms of Injury
November 04, 2021 | Location: On-Demand, Virtual Only
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Menshikh, Anna, Vanderbilt University Medical Center, Nashville, Tennessee, United States
- Delgado, Rachel, Vanderbilt University Medical Center, Nashville, Tennessee, United States
- Brewer, Maya, Vanderbilt University Medical Center, Nashville, Tennessee, United States
- Yang, Min, Vanderbilt University Medical Center, Nashville, Tennessee, United States
- Kennedy, Chris R., University of Ottawa, Ottawa, Ontario, Canada
- Yang, Haichun, Vanderbilt University Medical Center, Nashville, Tennessee, United States
- de Caestecker, Mark P., Vanderbilt University Medical Center, Nashville, Tennessee, United States
Background
Patients with diabetic kidney disease (DKD) are at increased risk of severe AKI and adverse outcomes. Since DKD may alter cellular and therapeutic responses in AKI, there is a need to establish a model to evaluate the mechanisms and therapeutics of AKI in the context of DKD. These studies address this deficiency by characterizing AKI in transgenic mice with renin-induced hypertension and progressive DKD.
Methods
Hypertensive male transgenic TThRen (TR+) mice, which over-express renin in the liver, were treated +/- streptozotocin to induce diabetes (DM). Tail cuff BP; urinary albumin/creatine ratio (ACR); BUN; and transdermal GFR (tGFR) were measured from 11-31 wks in DM and non-diabetic (ND) cohorts. After optimizing renal pedicle clamp times in DM (19 mins) vs. ND mice (24 mins) to induce similar injury after ischemia-reperfusion AKI (IR-AKI), mice underwent bilateral IR-AKI at 32 wks, followed by serial BP, BUN, ACR and tGFR measurements over 8 wks.
Results
ND and DM TR+ mice had increased albuminuria (31wk ACR, mg/mg: ND TR - vs. +, 17.7 (2.0) vs. 498.2 (325.2), p<0.0001; DM TR – vs. +, 136.7 (50.3) vs. 1028 (420.3) p<0.01), with no significant difference between DM and ND TR+ mice. BP was elevated in TR+ mice, with no difference between DM and ND mice, but there was a decrease in tGFR in DM TR+ vs. ND TR+ over time (2 way ANOVA, p<0.05). After IR-AKI, DM TR+ mice had more severe injury than DM TR- mice (e.g.: day1 BUN, mg/dl: DM TR- vs. +, 56.7 (8.6) vs. 95.1 (11.8), p<0.01), but there was no difference in severity of injury in ND TR- and + mice. This was associated with a progressive increase in albuminuria, but after 14 days, no difference in tGFR in DM TR- vs. + mice, and no change in albuminuria or tGFR in ND TR- vs. + mice up to 8 weeks after IR-AKI.
Conclusion
Hypertensive DM and ND mice develop albuminuria, but only hypertensive DM mice develop reduced tGFR consistent with progressive DKD. After optimizing renal pedicle clamp times to induce similar injury, only hypertensive DM mice had increased injury and progressive nephropathy after IR-AKI, as determined by increased albuminuria, compared with non-hypertensive DM and both hypertensive and non-hypertensive ND controls. This model mimics human disease as there is increased injury and progressive DKD after IR-AKI mice with pre-existing DKD.
Funding
- Other U.S. Government Support