Abstract: PO0357
The Effect of Ischemia-Reperfusion Injury on Nuclear-Reduced Glutathione Levels in Kidneys from Old Female Lewis Rats
Session Information
- AKI: Mechanisms of Injury
November 04, 2021 | Location: On-Demand, Virtual Only
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Zamlauski-Tucker, Marianna J., Ball State University, Muncie, Indiana, United States
- Ye, Bingwei, Ball State University, Muncie, Indiana, United States
Background
The purpose of the study was to determne the effect of ischemia/reperfusion injury (IRI) on nuclear reduced glutathione (GSH) levels in kidneys from old rats. GSH is the major antioxidant inside cells, and a decrease in GSH levels would contribute to the damage caused by free radicals that are elevated in IRI. There is limited information on the effect of IRI on nuclear GSH levels.
Methods
Anesthetized old female Lewis rats (22 months of age) were used in the study. The left and right renal pedicles was clamped for 60 min, followed by 60 min of reperfusion in the Experimental Group (n=5). The kidneys were then harvested, separated into cortex and medulla, and homogenized. Kidneys in the Control Group (n=5) were not subjected to IRI before being harvested. The nuclear fractions were isolated using differential centrifugation, and GSH levels were measured using a spectrophotmetric assay. The water contents of the cortex and medulla were determined to allow GSH levels to be expressed as nmol/ g kidney dry weight. A Student's T test was used to compare the groups, and statistical significance was determined at p < 0.05. All data shown as X ± SEM.
Results
Nuclear GSH levels were significantly decreased in both the kidney cortex and medulla of the Experimental Group when compared to the Control Group. Nuclear GSH levels in the cortex decreased by 28%, with nuclear GSH being 535 ± 56 nmol/g kidney dry wt in the Control Group, and decreased to 385 ± 23 nmol/g kidney dry wt in the Experimental Group exposed to IRI. Nuclear GSH levels in the medulla decreased by 54%, with nuclear GSH being 676 ± 72 nmol/g kidney dry wt in the Control Group, and decreased to 309 ± 29 nmol/g kidney dry wt in the Experimental Group exposed to IRI.
Conclusion
After 60 min of ischemia, nuclear GSH levels in rat kidney cortex and medulla did not return to normal levels after 60 min of reperfusion. The results suggests that the nucleus is experiencing major oxidative stress and damage caused by free radicals in IRI, and this may be contributing to the renal dysfunction seen in IRI.