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Kidney Week

Abstract: PO0683

Ferroptosis Is Involved in the Process of Diabetic Kidney Disease

Session Information

Category: Diabetic Kidney Disease

  • 601 Diabetic Kidney Disease: Basic

Authors

  • Wu, Keping, Sun Yat-Sen University, Guangzhou, China
  • Fei, Lingyan, Sun Yat-Sen University, Guangzhou, China
  • Liu, Yang, Sun Yat-Sen University, Guangzhou, China
  • Chen, Jiasi, Sun Yat-Sen University, Guangzhou, China
  • Zhu, Enyi, Sun Yat-Sen University, Guangzhou, China
  • Guan, Hui, Sun Yat-Sen University, Guangzhou, China
  • Zhong, Ming, Sun Yat-Sen University, Guangzhou, China
  • Zheng, Zhihua, Sun Yat-Sen University, Guangzhou, China
  • Wang, Xiaohua, Sun Yat-Sen University, Guangzhou, China
Background

Diabetic kidney disease(DKD) is a major public health problem that threatens human health and causes substantial economic burden. DKD is accompanied by accumulation of ROS and iron in the kidney, a hallmark of ferroptosis.
Ferroptosis is a condition that causes cell death by accumulation of lipid reactive oxygen species (ROS), in an iron-dependent mechanism that is different from apoptosis, necroptosis and autophagy. That ferroptosis is involved in DKD has been shown recently, but its role is still unknown.

Methods

We induced diabetic kidney disease in 8-week-old male rats with streptozotocin (STZ) and treated with ferroptosis inhibitor Fer-1 to analyze the degree of renal injury and the related indexes of ferroptosis.

Results

1.Diabetic renal injury involves ferroptosis. Accumulation of iron was also confirmed by Prussian blue staining and presented morphological changes linked to ferroptosis in DKD group: reduced mitochondrial volume, ruptured mitochondrial membrane and missing mitochondrial cristae.
2.Blocking ferroptosis can alleviate proteinuria and renal tubular injury in STZ-induced DKD. Fer-1 treatment clearly decreased the urine protein-creatinine ratio, both α-1 microglobulin and N-acetyl-β-D-glucosaminosidase in DKD group and the levels of both MDA and Fe2+. In addition, treating DKD rats with Fer-1 reduced iron in the kidney and alleviated kidney fibrosis.

Conclusion

Ferroptosis is involved in the process of diabetic kidney disease.

Markers of renal tubular injury α 1-microglobulin and N-acetyl-β-D-glucosaminosidase measured in urine