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Abstract: PO1941

Time-Course Kidney Injury in Mice Remnant Kidney Model Fed by High-Protein Diet

Session Information

Category: Pathology and Lab Medicine

  • 1600 Pathology and Lab Medicine

Authors

  • Tanaka, Shohei, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
  • Wakui, Hiromichi, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
  • Urate, Shingo, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
  • Suzuki, Toru, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
  • Abe, Eriko, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
  • Tsukamoto, Shunichiro, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
  • Taguchi, Shinya, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
  • Azushima, Kengo, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
  • Tamura, Kouichi, Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan
Background

Numerous animal models of CKD have been developed, but mice are relatively resistant to kidney injury. The remnant kidney model mimics progressive renal failure, and widely used in CKD research. The present study was performed to evaluate the effects of combined high-protein diet (HPD) loading and 5/6 nephrectomy (Nx) in a susceptible strain of mice (129/Sv).

Methods

Male 8–11-week-old 129/Sv mice underwent 5/6 Nx or sham surgery, then 2 weeks later were switched to an HPD, and cardiovascular parameters, kidney function, and renal histology were assessed after 4, 8, or 12 weeks.

Results

The 5/6 Nx group showed blood pressure elevation, cardiac hypertrophy, renal function decline, severe albuminuria, and glomerular hypertrophy. However, the glomerulosclerosis by 5/6 Nx was very mild and there was only modest tubulointerstitial inflammation and fibrosis in the 5/6 Nx group, even after 12 weeks of HPD loading. Furthermore, the sham group showed no histological changes.

Conclusion

Thus, an HPD alone is insufficient to cause renal pathology, and a combination of 5/6 Nx and HPD loading induces mild renal pathology.