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Kidney Week

Abstract: PO2181

Lymphocyte Subpopulations in Clinical Practice After Kidney Transplantation: B Cell Levels Predict Renal Function at 1 Year

Session Information

Category: Transplantation

  • 1902 Transplantation: Clinical

Authors

  • Comai, Giorgia, 1) Nephrology, Dialysis and Kidney Transplantation Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
  • Corradetti, Valeria, 1) Nephrology, Dialysis and Kidney Transplantation Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
  • Vischini, Gisella, 1) Nephrology, Dialysis and Kidney Transplantation Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
  • Maritati, Federica, 1) Nephrology, Dialysis and Kidney Transplantation Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
  • Bini, Claudia, 1) Nephrology, Dialysis and Kidney Transplantation Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
  • La Manna, Gaetano, 1) Nephrology, Dialysis and Kidney Transplantation Unit, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Bologna, Italy
Background

Lymphocytes subpopulations play a key role in the immune response after kidney transplantation. Many different T-lymphocyte have been studied for their suitability to monitor allograft rejection. B cells have been associated with acute and chronic antibody mediated rejection and with poor outcome after transplant but are associated with tolerant kidney transplant recipients.

Methods

We retrospectively analyze the lymphocyte subpopulation (total lymphocyte, CD3+, CD4+, CD8+, NK, CD20+) pre transplant, after 1 week, at discharge and after 2 months post transplant in a cohort of kidney tranpslant recipients and we evaluate the impact of this subsets on kidney outcome.

Results

187 kidney transplant recipients were included in the study and a total of 748 samples were analyze. We didn't find any association between lymphocyte subsets and delayed graft function, primary non function and graft rejection. We found an association between low level of B lymphocyte at 2 months and 1-year GFR less than 45 ml/min (Figure 1 and 2). Multivariate analysis including donor age, cold ischemia time, rejection, recipient age, induction theraphy and steroid withdrawal confirm these finding.

Conclusion

Low level of B lymphocyte at 2 months post transplant is associated with poor graft oucome suggesting that these finding may predict a worse renal function at 1 year. The changes in lymphocyte subset after transplant could represent an early outcome marker usefull to personalized immunosuppressive therapy.