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Abstract: PO1933

Assessment of Glomerular Number in Fresh Renal Tissue and Renal Pathological Specimens

Session Information

Category: Pathology and Lab Medicine

  • 1600 Pathology and Lab Medicine


  • Sonoda, Kosuke, Shinshu Daigaku, Matsumoto, Nagano, Japan
  • Harada, Makoto, Shinshu Daigaku, Matsumoto, Nagano, Japan
  • Hashimoto, Koji, Shinshu Daigaku, Matsumoto, Nagano, Japan
  • Kamijo, Yuji, Shinshu Daigaku, Matsumoto, Nagano, Japan

On-site evaluation of fresh renal tissue at the time of renal biopsy is useful. However, some cases present poor correlation in glomerular number between fresh renal tissue and renal pathological specimens.


To examine the usefulness of on-site evaluation, the correlation between glomerular number in fresh renal tissue and renal pathological specimens, and associated factors disturbing the evaluation were investigated via a retrospective cross-sectional observational study.


In the included 129 cases, there was a significant positive correlation between glomerular number in fresh renal tissue and renal pathological specimens. The median ratio of glomerular number (renal pathological specimen/fresh renal tissue) was 0.74 (0.48–0.97). According to this ratio, all cases were divided into three groups: a reasonable estimation group (65 cases), underestimation group (32 cases), and overestimation group (32 cases). Comparing the reasonable estimation group with the underestimation group, significant differences were detected in the extent of interstitial fibrosis and tubular atrophy (IFTA) and in the extent of interstitial inflammation. Logistic regression analyses also demonstrated that IFTA and interstitial inflammation were significantly associated with underestimation.


In conclusion, glomerular number counted by on-site evaluation of fresh renal tissue estimated the actual number of glomeruli in the renal pathological specimen, suggesting clinical benefit. Since tubulointerstitial lesions, such as IFTA and/or interstitial inflammation, may make it difficult to recognize glomeruli in fresh renal tissue, the possibility of underestimation of results for cases with possible severe tubulointerstitial lesions should be considered.