Abstract: PO0342
Evaluation of Urinary NHE3 in Rats with AKI
Session Information
- AKI: Mechanisms of Injury
November 04, 2021 | Location: On-Demand, Virtual Only
Abstract Time: 10:00 AM - 12:00 PM
Category: Acute Kidney Injury
- 103 AKI: Mechanisms
Authors
- Xie, Anni, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
- Ren, Zhiyun, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
- Liu, Mingda, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
- Yu, Yanting, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
- Wang, Xiaoyan, The Core Laboratory,Nanjing BenQ Medical Center,The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
Group or Team Name
- The Core Laboratory for Clinical Research
Background
Acute kidney injuries (AKIs), caused by hypovolemia, ischemia-reperfusion, or nephrotoxins , are concerned with high morbidity and mortality. Urinary Na/H exchanger isoform 3 (NHE3) has been demonstrated as a noninvasive marker of acute tubular necrosis. However, the ideal diagnostic biomarker in AKI is still lacking.
Methods
In order to determine the potential role of urinary NHE3 in early diagnosis of AKI,we evaluated the exosome NHE3 in daily urines from rat models of AKIs including low NaCl (0.01%) plus candesartan (1mg/kg/day, IP) for 7 days, ischemia/reperfusion (ischemia for 40min,referfusion for 2h）and cisplatin(20mg/kg for 7 days) in Sprague Dawley rats (male, 2-3 months old, 4-7 rats per group). Urine exosomes were isolated by a series of centrifuges including ultracentrifuges (17k xg,4°C,10min;200k xg,4°C,1 hr).
Results
NHE3 levels (western blotting) were increased at day 1, which was 1 day before serum creatinine increased in low NaCl/candesartan rats and reperfusion rats (day1) relative to controls. They were also increased in cisplatin rats at day 2 (1 day before serum creatinine increased). Furthermore, NHE3 in original urines from 6 patients diagnosed with AKI ( Scr 249.83 ±166.93 umol/L) and 6 volunteers with normal renal function (Scr 68.67±13.20umol/L) were assessed without ultracentrifuge isolation. NHE3 was increased remarkably in AKI patients (333±28, % of controls) compared with volunteers (100±30, %, t-test, P<0.05).
Conclusion
Our results in rats and patients suggest that assessment of urine NHE3 may be a potential non-invasive biomarker for early detection of various acute kidney injuries.
Funding
- Government Support – Non-U.S.