Abstract: PO0742
The Potential Value of Urinary Extracellular Vesicles VEGF-A165b in Diagnosis of Diabetic Kidney Disease
Session Information
- Diabetic Kidney Disease: Clinical
November 04, 2021 | Location: On-Demand, Virtual Only
Abstract Time: 10:00 AM - 12:00 PM
Category: Diabetic Kidney Disease
- 602 Diabetic Kidney Disease: Clinical
Authors
- Jing, Jing, Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, Jiangsu, China
- Feng, Songtao, Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, Jiangsu, China
- Lv, Linli, Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, Jiangsu, China
Background
Novel biomarkers are needed for management of diabetic kidney disease (DKD). Urinary extracellular vesicles (uEVs) were served as an ideal resource of biomarkers in kidney disease. VEGF-A165b is a angiogenic factor secreted from podocytes correlated with DKD. The study was aimed to evaluate the diagnostic value of uEVs-VEGF-A165b in DKD.
Methods
Urine samples were collected from 36 patients with T2DM and 12 controls. Subjects with T2DM were stratified into three groups according to UACR, eGFR, and T2DM duration. To isolate exosomes, 25 ml urine was ultracentrifuged to obtain exosomes. Protein was extracted from uEVs and subjected to western blot (Wb) for detecting VEGF-A165b. Immunohistochemistry (IHC) staining of VEGF-A165b was performed in kidney paraffin sections from STZ-induced DM rats. ROC curve was used to evaluate the diagnostic value of uEVs-VEGF-A165b in DKD.
Results
Urinary MVs and exosomal VEGF-A165b were higher in T2DM with ACR>300mg/g than those with ACR<30mg/g. In addition, urinary MVs VEGF-A165b were higher in patients with ACR30-300mg/g than those with ACR<30mg/g, and exosomal VEGF-A165b levels were lower in patients with ACR30-300mg/g than whose ACR>300 mg/g. Furthermore,VEGF-A165b in uEVs increased with the DM duration. VEGF-A165b in patients with duration longer than 10 years were higher than whose duration less than 5 years. Correlation analysis revealed eGFR was negatively correlated with urinary MVs and exosomal VEGF-A165b. ROC curve showed that AUC of urinary MVs and exosomal VEGF-A165b for the diagnosis of DKD were 0.9091 and 0.8269. IHC revealed that VEGF-A165b was elevated in renal tubules in STZ-induced DM rats.
Conclusion
A increased level of uEVs-VEGF-A165b was observed in DKD patients and was correlated with decline of eGFR. uEVs-VEGF-A165b may be used as a promising biomarker reflecting the severity of DKD and may suggest a pathological role in the development of the disease.