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Abstract: PO1797

Microvascular Endothelial Dysfunction in Women Living with HIV Represents Premature Aging

Session Information

Category: Hypertension and CVD

  • 1402 Hypertension and CVD: Clinical, Outcomes, and Trials

Authors

  • Wang, Dan, Georgetown University Medical Center, Washington, District of Columbia, United States
  • Wilcox, Christopher Stuart, Georgetown University Medical Center, Washington, District of Columbia, United States
Background

Combined antiretroviral therapy has permitted HIV-infected subjects to survive to old age yet many develop premature cardiovascular disease (CVD) characteristic of small vessel dysfunction though the causes are unclear. We reported that young HIV-infected individuals had endothelial dysfunction in their subcutaneous microarterioles (SMAs) dissected from a gluteal biopsy. We now test the hypothesis that this represents premature microvascular aging due to inflammation and reactive oxygen species (ROS).

Methods

SMAs from young (n=24) and old (n=18) virally-suppressed, HIV-infected and propensity-matched young (n=18) and old (n=16) HIV-negative controls were preconstructed on a myograph and relaxed with acetylcholine (Ach) to assess endothelium-dependent relaxation factor (EDRF). Circulating IL-6 and urinary 8-isoprostane F2α were measured to access markers of inflammation and ROS.

Results

Young HIV infected (vs uninfected) subjects had significantly (p< 0.05) reduced EDRF (26±3% vs 39±2%) and significantly (p<0.05) increased circulating IL-6 (13±2 vs 6±2 pg/ml ) and urinary 8-isoprostane F2α (2.7±0.5 vs 1.3±0.3 ng/ ml.mg creatinine). Amongst controls, EDRF was reduced significantly (p< 0.05) in old vs young (26±2 % vs 38±3 %), accompanied by increased IL-6 (15±3 vs 6 ± 1 pg/ml) and 8-isoprostane F2α (3.4±0.5 vs 1.3± 0.3 pg/ml). These variables were not significantly different between young and old HIV-infected individuals such that, amongst the old subjects, there was no difference (P=NS) between HIV infected (vs uninfected) subjects for EDRF, IL-6 or 8-isoprostane F2α.

Conclusion

Young subjects living with HIV have inflammation and ROS that impair their endothelial function but normal subjects develop age-dependent endothelial dysfunction sufficiently to abolish any difference with subjects who have HIV. Thus, microvascular disease in HIV represents the effects of inflammation and oxidative stress to cause premature aging.

Funding

  • Other NIH Support