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Abstract: FR-PO712

Consensus Draft of the Mouse Podocyte-Ome

Session Information

Category: Glomerular Diseases

  • 1304 Glomerular Diseases: Podocyte Biology

Authors

  • Hutzfeldt, Arvid D., Universitatsklinikum Hamburg-Eppendorf, Hamburg, Hamburg, Germany
  • Tan, Yifan, Aarhus Universitet, Aarhus, Midtjylland, Denmark
  • Bonin, Léna Lydie, Aarhus Universitet, Aarhus, Midtjylland, Denmark
  • Lassé, Moritz, Universitatsklinikum Hamburg-Eppendorf, Hamburg, Hamburg, Germany
  • Demir, Fatih, Aarhus Universitet, Aarhus, Midtjylland, Denmark
  • Rinschen, Markus M., Universitatsklinikum Hamburg-Eppendorf, Hamburg, Hamburg, Germany
Background

We aim to establish a common, simplified knowledgebase for the mouse "podocyte-ome" by integrating bulk RNA sequencing and bulk proteomics of sorted podocytes and single cell transcriptomics.

Methods

Three datasets of each omics type from three different laboratories, respectively, were integrated, visualized and bioinformatically analyzed.

Results

The procedure sheds light on conserved processes of podocytes, but also on limitations and specific features of the used technologies. High expression of glycan GPI anchor synthesis and turnover, and retinol metabolism was identified as a relatively understudied features of podocytes, while there are both podocyte-enriched and podocyte-depleted actin binding molecules. We compiled aggregated data in an application that illustrates the features of the dataset and allows for exploratory analyses through individual gene query of podocyte identity in absolute and relative quantification towards other glomerular cell types, keywords, GO-terms and gene set enrichments.

Conclusion

This consensus draft is a first step towards common molecular omics knowledge of kidney cells.

Funding

  • Government Support – Non-U.S.