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Abstract: FR-PO729

Analysis of GR and miRs Expression in Renal Tissues of Patients With Renal Diseases Compared to Controls

Session Information

Category: Glomerular Diseases

  • 1304 Glomerular Diseases: Podocyte Biology

Authors

  • Angelousi, Anna, Ethniko kai Kapodistriako Panepistemio Athenon Iatrike Schole, Athens, Attica, Greece
  • Liapis, Georgios, Ethniko kai Kapodistriako Panepistemio Athenon Iatrike Schole, Athens, Attica, Greece
  • Gazouli, Maria, Ethniko kai Kapodistriako Panepistemio Athenon Iatrike Schole, Athens, Attica, Greece
  • Kofotolios, Ioannis, Ethniko kai Kapodistriako Panepistemio Athenon Iatrike Schole, Athens, Attica, Greece
  • Boletis, Ioannis, Ethniko kai Kapodistriako Panepistemio Athenon Iatrike Schole, Athens, Attica, Greece
  • Marinaki, Smaragdi, Ethniko kai Kapodistriako Panepistemio Athenon Iatrike Schole, Athens, Attica, Greece
Background

Glucocorticoid Receptor (GR) is expressed in normal renal podocytes however, its expression differs among renal diseases. In this study, we analysed GR expression in renal tissues of patients with systemic lupus nephritis (LN), minimal changes disease (MCD) and pauci-immune glumeronephritis (PIN) as well as the expression of its epigenetic regulators miR30a, miR24 and miR370.

Methods

Fifty-one patients (n=20 with LN, n=14 with MCD and n=17 with PIN) underwent renal biopsy and 22 controls with no history of parenchymal renal disease were recruited from the Clinic of Nephrology and Renal Transplantation of General Laikon hospital through November 2016 to March 2019. All patients were recently diagnosed and were naïve of any treatment. mRNA and protein expression was analysed through real time -polymerase chain reaction (rt-PCR) and immunohistochemistry respectively. Data were collected after obtaining written consents forms from all participants.

Results

GR mRNA expression was underexpressed in all pathological specimens of patients compared to “normal” renal tissues of controls (p=0.023 for LN, p=0.05 for MCD and p=0.004 for PIN). Similarly, GR protein expression was lower in all pathological specimens (>6 GR positive podocytes/glomerulus in 42% of patients with LN, 55% with MCD and 16.68 % with PIN) compared with controls (> 6 positive podocytes/glomerulus in all the controls). PIN specimens presented also significantly lower GR mRNA and protein expression comparing with LN and MCD specimens among patients. No significant differences were found in the miR30a expression comparing pathological renal specimens of patients with “normal” renal tissues of controls. miR24 and miR370 expression demonstrated statistically significant difference in all pathological compared to “normal” tissues. miRs expression didn’t differ among the 3 studied groups. GR expression was not also associated neither with LN disease activity score nor with eGFR.

Conclusion

GR and miR24 expression was significantly underexpressed whereas miR370 significantly overexpressed in all pathological compared with adjacent “normal” renal tissues implying their potentional role in nephritis pathogenesis and treatment.