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Abstract: TH-PO254

The Effect of Diabetes in Morbidly Obese Patients on Protein Profiles of Circulating Extracellular Vesicles Before and After Bariatric Surgery

Session Information

Category: Diabetic Kidney Disease

  • 602 Diabetic Kidney Disease: Clinical

Authors

  • Lee, Haekyung, Soonchunhyang University Hospital, Yongsan-gu, Seoul , Korea (the Republic of)
  • Cho, Junghyun, Soonchunhyang University Hospital, Yongsan-gu, Seoul , Korea (the Republic of)
  • Chung, Eui Suk, Soonchunhyang University Hospital, Yongsan-gu, Seoul , Korea (the Republic of)
  • Kim, Hyoungnae, Soonchunhyang University Hospital, Yongsan-gu, Seoul , Korea (the Republic of)
  • Jeon, Jin seok, Soonchunhyang University Hospital, Yongsan-gu, Seoul , Korea (the Republic of)
  • Kwon, Soon hyo, Soonchunhyang University Hospital, Yongsan-gu, Seoul , Korea (the Republic of)
Background

Obesity and diabetes are often associated with cardiometabolic diseases, with substantial prognostic heterogeneity. We sought to identify proteomic signatures of circulating extracellular vesicles (EVs) of obesity with or without diabetes before and after bariatric surgery.

Methods

Circulating EV proteins were analyzed before and 6 months after bariatric surgery in 30 morbidly obese patients with (n = 12) or without (n = 18) diabetes and compared to those in 37 healthy volunteers.

Results

Bariatric surgery induced weight loss and improvements in fasting glucose, but urine albumin (p <0.01) decreased only in non-diabetic obese patients. A total of 26 and 271 EV proteins were significantly differentially expressed in diabetic obese patients compared to non-diabetic obese patients before and after bariatric surgery, respectively. Expression of proteins involved in the complement system and immunoglobulin-mediated immune response was upregulated in both non-diabetic and diabetic obese patients, whereas that of proteins involved in leukocyte- and lymphocyte-mediated immunity was downregulated in both groups after surgery (Figure 1). Expression of proteins involved in glycosylphosphatidylinositol phospholipase D activity and lipid catabolism was downregulated in diabetic obese patients, whereas that was upregulated in non-diabetic obese patients following surgery.

Conclusion

Diabetic obese patients have distinct EV protein signatures before and after bariatric surgery compared with non-diabetic obese patients. These obesity- and diabetes-specific EV protein expression profiles and their associated pathways may provide insight into the mechanisms responsible for the development of diabetes in obese patients.

Figure 1. Functional interpretation of significantly altered extracellular vesicle proteins after bariatric surgery