Kidney Week

Abstract: TH-PO495

Gut Microbiota Profiles in IgA Nephropathy Patients and Their Household Members

Session Information

• Glomerular Diseases: Clinical, Outcomes, Trials - I
  November 03, 2022 | Location: Exhibit Hall, Orange County Convention Center‚ West Building
  Abstract Time: 10:00 AM - 12:00 PM

Category: Glomerular Diseases

• 1303 Glomerular Diseases: Clinical‚ Outcomes‚ and Trials

Authors

• Kim, Yaerim, Keimyung University School of Medicine, Daegu, Korea (the Republic of)

Yaerim Kim,

• Park, Sehoon, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)

Sehoon Park,

• Paek, Jin hyuk, Keimyung University School of Medicine, Daegu, Korea (the Republic of)

Jin hyuk Paek,

• Park, Woo Yeong, Keimyung University School of Medicine, Daegu, Korea (the Republic of)

Woo Yeong Park,

• Jin, Kyubok, Keimyung University School of Medicine, Daegu, Korea (the Republic of)

Kyubok Jin,

• Han, Seungyeup, Keimyung University School of Medicine, Daegu, Korea (the Republic of)

Seungyeup Han,

• Lee, Jung Pyo, Seoul National University Seoul Metropolitan Government Boramae Medical Center, Dongjak-gu, Seoul, Korea (the Republic of)

Jung Pyo Lee,

• Kim, Dong Ki, Seoul National University Hospital, Jongno-gu, Seoul, Korea (the Republic of)

Dong Ki Kim,

Background

Although immunoglobulin A nephropathy (IgAN) is not a definite genetic disease, it has an autoimmune trait of complex architecture with a solid genetic predisposition. Herein, we aimed to evaluate the difference in microbiota profile among patients with IgAN, their household members and unrelated healthy controls.

Methods

We prospectively recruited subjects with IgAN and their household member and healthy control between July 2019 and August 2021. Gut microbiota was analyzed using the Illumina MiSeq system based on the 16S rRNA gene. We compared the abundance of the microbiome in each level of phylum, class, order, family, and genus between the groups using Mann–Whitney U test. Also, we tried to find a specific genus representing the disease severity based on the Oxford classification.

Results

A total of 73 subjects (IgAN 45, household member 14, healthy control 14) were finally included in the study. The mean age of each group was 38.2, 44.0, and 32.3 years old in IgAN, household members, and healthy controls, respectively. The mean eGFR was 97.6, 101.5, and 113.7 mL/min/1.73 m², respectively. The primarily observed phylum was Actinobacteria, Firmicutes, and Bacteroidetes. Among Firmicutes phylum, Blautia and Anaerobutyricum were found to be significantly higher in subjects with IgAN (13.7% and 4.1%) than in household members (9.2% and 1.5%) and healthy controls (8.6% and 2.1%). The abundance of Dorea was higher in IgAN than a household member, but not than healthy control. On the contrary, Prevotella and Ruminococcus were higher in IgAN than healthy control, not a household member. Bifidobacterium was significantly lower in M1, but Clostridium was higher in M1. Holdemanella and Coprococcus were significantly higher in E1. Prevotella and Coprococcus were higher in S1, but Streptococcus was lower in S1. There was no identified genus to distinguish T and C scores.

Conclusion

The gut microbiota was well discriminated subjects with IgAN from household members and healthy controls. In addition, it was also differently observed according to the status of pathologic findings of IgAN. These results may provide a basis for further metagenomics analysis using a family cohort of IgAN.