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Abstract: FR-PO058

A Novel Contrast-Induced AKI Mouse Model Based on Low-Osmolar Contrast Medium

Session Information

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Shen, Jianxiao, Renji Hospital, Shanghai, China
  • Ni, Zhaohui, Renji Hospital, Shanghai, China
Background

The contrast-induced acute kidney injury (CI-AKI) has been becoming the third common cause of hospital-acquired acute kidney injury.
An ideal animal model is essential for understanding the pathophysiology of CI-AKI.
This study provides a novel, efficient and reproducible CI-AKI model which was developed in mouse by administrating a low-osmolar contrast medium (LOCM).

Methods

First, we applied the frequently-used pretreatments (uninephrectomy and water deprivation), which combined with HOCM on rats could induce CI-AKI, on mice with LOCM.
Secondly,we attempted to find a novel preatment suitable for mouse and LOCM by combining two classic pretreatments(uninephrectomy, water deprivation and furosemide administration).
Finally, we evaluate the kidney damage of the novel model.

Results

This mouse model possessed a significant reduction in renal function, severe renal tissue damage, and increased renal tubular cells apoptosis, indicating that LOCM is a feasible inducer for CI-AKI mice model.

Conclusion

We found that uninephrectomy(UPHT) combined with 24h water deprivation and furosemide administration 20 min before LOCM (iohexol, 10 ml/kg) application is a feasible pretreatment to establish a novel CI-AKI mouse model.

Funding

  • Other NIH Support