Kidney Week

Abstract: TH-PO795

Disruption of Crb2 in Podocytes After Birth Leads to Proteinuria

Session Information

• Genetic Diseases of the Kidney - I
  November 07, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: Genetic Diseases of the Kidneys

• 1002 Genetic Diseases of the Kidneys: Non-Cystic

Authors

• Tanoue, Akiko, Mie university graduate school of medicine, Tsu, Japan

Akiko Tanoue,

• Katayama, Kan, Mie university graduate school of medicine, Tsu, Japan

Kan Katayama,

• Oda, Keiko, Mie university graduate school of medicine, Tsu, Japan

Keiko Oda,

• Fujimoto, Mika, Mie University Graduate School of Medicine, Mie, Japan

Mika Fujimoto,

• Hirabayashi, Yosuke, Mie university graduate school of medicine, Tsu, Japan

Yosuke Hirabayashi,

• Mizoguchi, Shoko, Mie university graduate school of medicine, Tsu, Japan

Shoko Mizoguchi,

• Haruki, Ayumi, Mie University, Tsu, Japan

Ayumi Haruki,

• Ito, Takayasu, Mie University Graduate School of Medicine, Mie, Japan

Takayasu Ito,

• Murata, Tomohiro, Mie university graduate school of medicine, Tsu, Japan

Tomohiro Murata,

• Yokoi, Hideki, Kyoto University Graduate School of Medicine, Kyoto City, Japan

Hideki Yokoi,

• Ito, Masaaki, Mie University Graduate School of Medicine, Mie, Japan

Masaaki Ito,

Background

Crumbs 2, Crb2 is one of the components of the Crumbs cell polarity complex and known to be expressed in the podocytes. There have been reports that CRB2 mutations were associated with steroid-resistant nephrotic syndrome in human. However, its precise mechanism is still unclear.

Methods

Crb2 floxed mice that had loxP sites flanking exon 7 and 8 were bred with NPHS2-Cre ERT2 mice to generate NPHS2-Cre ERT2 positive Crb2 floxed mice. Podocyte-specific Crb2 knockout mice after birth were made by injecting tamoxifen or negative control intraperitoneally for three days at two months of age. Urine, blood, and kidneys were analyzed in the two mouse groups at four months of age.

Results

NPHS2-Cre ERT2 positive Crb2 floxed mice that were injected tamoxifen showed massive proteinuria at four months of age compared to those that were injected negative control. Blood urea nitrogen or serum creatinine at four months of age was comparable between the two groups. Glomerular indices or fibrotic indices at four months of age were similar between the two groups.

Conclusion

Disruption of Crb2 in the podocytes after birth is related to inducing proteinuria.

Funding

• Private Foundation Support