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Abstract: FR-PO955

The Differential Expression Research of Circular RNAs in Exosomes from Serum and Urine in Systemic Lupus Erythematosus Patients

Session Information

Category: Pathology and Lab Medicine

  • 1601 Pathology and Lab Medicine: Basic

Author

  • Ma, Hualin, Shenzhen People's Hospital, Shenzhen, Guangdong, China
Background

To further explore the pathogenesis of SLE, the technique of gene-sequencing was used to analyze the differentially expressed circular RNAs (circRNAs) in exosomes both from serum and urine of patients with SLE, which may lay the foundation for the research of circRNAs as a new class of exosome-based SLE diagnosis biomarkers.

Methods

Ten patients with SLE (SLE group) and ten normal controls (NC group) were recruited as experimental subjects in our research. The serum and urine were separated from each participant’s peripheral venous blood and early morning urine, which the exosomes were extracted from the collected serum and urine by the ExoQuick Exosome Precipitation Solution and ultracentrifugation. Then the pure circRNAs were extracted from the exosomes with a series of enzymatic reactions. And then, the significantly differentially expressed circRNAs were picked out by the method of gene-sequencing to analyze the function of corresponding target genes.

Results

Compared with normal controls, the species of circRNAs were reduced in the exosomes from serum of patients with SLE, which were mostly originated from intron gene regions; Meanwhile, a total of 121 circRNAs were significantly differentially expressed, which were also mostly derived from intron gene regions, including 54 up-regulated and 67 down-regulaed. But the species were increased in the exosomes from urine of patients with SLE compared with normal controls, and which were mainly originated from intron gene regions; Simultaneously, a total of 14 circRNAs were significantly differentially expressed, which were primarily belonged to intron gene regions, including 7 up-regulated and 7 down-regulaed. Compared with the circRNAs detected from urinary exosomes, a total of 52 circRNAs were significantly differentially expressed in the exosomes from serum of patients with SLE, which were also mostly originated from intron gene regions, including 45 up-regulated and 7 down-regulaed.

Conclusion

The significantly differential and specific expression of circRNAs in the exosomes from serum and urine of patients with SLE were found. Such as gene snoU13, SNORA31 and SNORA51 could be regarded as potential diagnostic biomarkers of SLE. Furthermore,these figures suggested that the significantly differentially expressed circRNAs can be used as a reference or a supplement in the research of the pathogenesis of SLE.