Abstract: SA-PO828
Protective Effect of Leuconostoc in Renal Damage Induced by Obesity
Session Information
- Health Maintenance, Nutrition, Metabolism - II
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Health Maintenance, Nutrition, and Metabolism
- 1300 Health Maintenance, Nutrition, and Metabolism
Authors
- Sanchez-Navarro, Andrea, Molecular Physiology Unit IIB/UNAM, México, Mexico
- Castro, Diana Catalina, Instituto Nacional de Ciencias Medicas y Nutricion SZ, Mexico City, Mexico
- Pérez-villalva, Rosalba, Molecular Physiology Unit IIB/UNAM, México, Mexico
- Martinez-Rojas, Miguel Angel, Molecular Physiology Unit IIB/UNAM, México, Mexico
- Zambrano, Elena, Instituto Nacional de Ciencias Medicas y Nutricion SZ, Mexico City, Mexico
- Bobadilla, Norma, Molecular Physiology Unit IIB/UNAM, México, Mexico
Background
There is enough evidence showing that obesity promotes kidney damage. Probiotics might help in improving several diseases. Mexican honey water contains bacteria known as Leuconostoc that have anti-obesity activity. However, its role in prevention of renal injury induced by obesity still unknown.
Methods
Forty three C57BL6 male mice were divided into four groups: control diet, 3.41 Kcal/g (n=10), probiotic control, 2x109 CFU, IG, (n=10), hypercaloric diet, 4.9 Kcal/g, (n=11), and hypercaloric diet+probiotic (n=12). The groups were studied for 3 months and a half. Weekly measurements of weight and caloric intake were made. At the end of the follow-up, the determination of body fat and water was carried out using nuclear magnetic resonance (NMR). Urine was collected to analyze renal function and biomarkers. Tissue was stored for molecular analysis and the other kidney was fixed for histopathological analysis.
Results
At the end of the study, the hypercaloric diet induced a significant increase by 52.1% in body weight (BW) and by 3-fold in body fat (BF), with a reduction in lean mass by 27.4%. Also renal damage was observed that was characterized by a significant increase in: albuminuria, oxidative stress and KIM-1 levels, as well as, kidney inflammation, and TGF-b up-regulation. Leuconostoc administration significantly reduced the increase in BW and BF by 14.2% and 42.8%, respectively, with the same food intake. Interestingly, albuminuria, oxidative stress, and KIM-1 levels in the hypercaloric+probiotic group were similar to control groups. Effects that were accompanied by restoration of IL-6, TNFa, and TGF-b mRNA levels.
Conclusion
These results show that hypercaloric diet induced metabolic and renal alterations. While, Leuconostoc was efficient in preventing weight gain, renal injury, and oxidative stress, as well as, profibrotic and inflammatory pathways activation. Therefore, this probiotic seems to be a feasible and useful tool for the treatment of renal injury induced by obesity; however, controlled clinical studies are required to prove this hypothesis.
Funding
- Government Support - Non-U.S.