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Abstract: TH-PO322

Hemodynamic and Geometrical Characteristics of Rat Arteriovenous Fistula: Effect of Nitric Oxide Treatment

Session Information

  • Vascular Access - I
    November 07, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: Dialysis

  • 704 Dialysis: Vascular Access

Authors

  • Northrup, Hannah M., University of Utah, Salt Lake City, Utah, United States
  • Shiu, Yan-Ting, University of Utah, Salt Lake City, Utah, United States
  • Pike, Daniel, University of Utah, Salt Lake City, Utah, United States
  • Falzon, Isabelle Dorothy, University of Utah, Salt Lake City, Utah, United States
  • Somarathna, Maheshika Srimali, University of Alabama at Birmingham, Birmingham, Alabama, United States
  • Guo, Lingling, University of Alabama at Birmingham, Birmingham, Alabama, United States
  • Lee, Timmy C., Univ of Alabama at Birmingham, Birmingham, Alabama, United States
Background

Nitric oxide regulates endothelial function and promotes vascular health. Overexpression of endothelial nitric oxide synthase has been shown to improve arteriovenous fistula (AVF) maturation in mice. AVF maturation is dependent on hemodynamics and remodeling of the vessel wall. Here we investigate the impact of localized delivery of nitric oxide on AVF lumen hemodynamics and geometry in rats.

Methods

Femoral AVFs were created in 12-16 week old male Sprague-Dawley rats. At the time of creation AVFs were treated with NO releasing nanomatrix gel therapy (treatment gels) (n=4). Gels without nitric oxide (placebo gels) were used as a control (n=3). 7 days post creation, animals were subject to non-contrast MRI scans, and the MR images were used for reconstruction of the AVF lumen and computational fluid dynamic simulations of the AVF blood flow. Hemodynamic parameters (velocity, fluid wall shear stress (FWSS), and vorticity) and geometrical analysis (anastomosis angle (AA), tortuosity, and nonplanarity angle (NA)) were calculated.

Results

As shown in Fig. 1, AVF vein velocity, FWSS, and vorticity of placebo gels were significantly higher than treatment gels (p<0.05). AA were not significantly different between placebo and treatment gels. Tortuosity of the AVF showed a higher trend in treatment gels than control gels, with NA showing a slight decrease in treatment gels.

Conclusion

The NO treatment leads to significantly lower hemodynamics than the placebo, suggesting the return of velocity, FWSS, and vorticity to the pre-surgery baseline levels in the native vein by NO. However, localized treatment of NO releasing nanomatrix gels does not result in significant differences in AVF lumen geometry.

Funding

  • NIDDK Support