Abstract: TH-PO323
Effect of Endothelial Nitric Oxide Synthase on Geometrical Parameters of Murine Arteriovenous Fistulas
Session Information
- Vascular Access - I
November 07, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 704 Dialysis: Vascular Access
Authors
- Falzon, Isabelle Dorothy, University of Utah, Salt Lake City, Utah, United States
- Shiu, Yan-Ting, University of Utah, Salt Lake City, Utah, United States
- Pike, Daniel, University of Utah, Salt Lake City, Utah, United States
- Northrup, Hannah M., University of Utah, Salt Lake City, Utah, United States
- Somarathna, Maheshika Srimali, University of Alabama at Birmingham, Birmingham, Alabama, United States
- Guo, Lingling, University of Alabama at Birmingham, Birmingham, Alabama, United States
- Lee, Timmy C., University of Alabama at Birmingham, Birmingham, Alabama, United States
Background
Arteriovenous fistula (AVF) maturation failure is a significant clinical issue. Endothelial nitric oxide synthase (NOS3) leads to the production of nitric oxide, a vasodilator, which contributes to successful AVF maturation in mice. Previous small clinical studies have reported the association between the AVF geometrical parameters and AVF maturation. Here we investigated the effect of NOS3 on AVF geometry in mice.
Methods
Carotid-jugular AVFs were created in NOS3 over expression (OE) and NOS3 knock out (KO) mice on C57BL/6 background, with C57BL/6 mice as wild type (WT) control (n=1 per strain). Black-blood MR images were taken at Day 7 and Day 21 post AVF creation and used to reconstruct AVF lumen geometries. Geometrical analysis (Fig. 1A) quantified the anastomosis angle (AA), nonplanarity angle magnitude (NA), and tortuosity of the AVF vein.
Results
The AVF lumen area was bigger in the NOS3 OE mice than in NOS3 KO and WT mice. Lumen reconstructions are shown in Fig. 1B. Overexpression of NOS3 led to a reduced AA and NA by 16° and 10°, respectively, from Day 7 to Day 21, indicating that the AVF vein remodeled to align more parallel with and on the same plain of the feeding artery. In contrast, NOS3 KO increased NA by 14° and decreased AA by 0.3° from Day 7 to Day 21. WT increased AA and NA by 17° and 5°, respectively, from Day 7 to Day 21. While OE and KO decreased in tortuosity by 0.15 and 0.14, respectively, WT increased tortuosity by 0.08 from Day 7 to Day 21.
Conclusion
Geometrical parameters differ with varying NOS3 expression and over time. More research is needed to understand how these geometrical parameters affect AVF maturation and the mechanisms leading to geometrical changes.
Funding
- NIDDK Support