Abstract: FR-PO272
Vitamin D Replacement Improves Renal Function, Hemodynamics, and Inflammatory and Morphological Parameters in Vitamin D-Deficient Rats After Renal Ischemia-Reperfusion Injury
Session Information
- CKD: Epidemiology and Risk Factors
November 08, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2101 CKD (Non-Dialysis): Epidemiology, Risk Factors, and Prevention
Authors
- de Braganca, Ana Carolina, Faculty of Medicine - University of Sao Paulo, São Paulo, São Paulo, Brazil
- Volpini, Rildo A., Faculty of Medicine - University of Sao Paulo, São Paulo, São Paulo, Brazil
- Canale, Daniele, Faculty of Medicine - University of Sao Paulo, São Paulo, São Paulo, Brazil
- Shimizu, Maria HM, Faculty of Medicine - University of Sao Paulo, São Paulo, São Paulo, Brazil
- Goncalves, Janaina Garcia, Faculty of Medicine - University of Sao Paulo, São Paulo, São Paulo, Brazil
- Seguro, Antonio C., Faculty of Medicine - University of Sao Paulo, São Paulo, São Paulo, Brazil
- Santos, Michele Santiago dos, Faculty of Medicine - University of Sao Paulo, São Paulo, São Paulo, Brazil
Background
The initial damage after an ischemia/reperfusion injury (IRI) plays an important role in the pathogenesis of acute kidney injury (AKI) and predisposition to CKD. Vitamin D deficiency (VDD) is associated with hemodynamic changes, activation of inflammatory pathaways and renal disease progression following IRI-AKI. Conversely, vitamin D sufficiency may be considered a protective factor. We evaluated the effect of vitamin D replacement (VR) in IRI rats under VDD.
Methods
We performed bilateral 45 min IRI on day 30 in all groups. Male Wistar rats were randomized into three groups: (1) IRI [fed a standard diet (SD) for 120 days]; (2) VDD+IRI [fed a vitamin D-free diet (D) for 120 days]; and (3) VDD+IRI+R (fed a D diet for 30 days and just after IRI, on day 31, we reintroduced the SD). We evaluated inulin clearance (Cin); mean arterial pressure (MAP); renal blood flow (RBF); renal vascular resistance (RVR); proteinuria; plasma levels of 25(OH)D and aldosterone as well as renal tissue levels of collagen 3 (COL3) and MCP1 by ELISA; and immunoblotted for VDR.
Results
VDD+IRI+R animals had 25(OH)D levels restored (~42 ng/mL). VR improved renal function and hemodynamic parameters. Also, decreased proteinuria and the amount of MCP1 and COL3 in renal tissue (Table 1).
Conclusion
Our study suggests that VR improved the recovery of renal fuction, hemodynamics, inflammatory and morphologycal features in IRI-AKI associated with VDD. Thus, vitamin D monitoring and replacement should be considered in renal patients. Financial Support: FAPESP 2018/04930-6, 2018/12297-1; CNPq 302599/2018-5.
Table 1
| IRI | VDD+IRI | VDD+IRI+R | |
| Cin (mL/min/100g BW) | 0.47±0.02 | 0.46±0.03 | 0.57±0.02cf |
| MAP (mmHg) | 124±2.5 | 142±3.7a | 122±2.5d |
| RBF (mL/min) | 7.9±0.43 | 6.0±0.03c | 8.8±0.49e |
| RVR (mmHg/mL/min) | 16.01±1.07 | 22.37±0.38a | 13.93±0.85d |
| Aldosterone (pg/mL) | 2172±328 | 3612±662c | 1753±167e |
| Proteinuria (mg/24 h) | 10.14±0.64 | 11.99±0.83 | 9.13±0.74f |
| VDR (%) | 100.8±3.9 | 58.9±3.6a | 148.3±5.5ad |
| COL3 (ng/ug protein) | 2.15±0.13 | 2.64±0.27 | 1.95±0.06f |
| MCP1 (pg/ug protein) | 2.82±0.25 | 3.76±0.38c | 2.81±0.15f |
Data are expressed as mean±SEM. BW: Body weight. a p<0.001, c p<0.05 vs IRI; d p<0.001, e p<0.01, f p<0.05 vs VDD+IRI.
Funding
- Government Support - Non-U.S.