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Abstract: FR-PO997

Imaging Renal Inflammation and Fibrosis After Ischemia-Reperfusion Injury by Novel Diffusion MRI

Session Information

Category: Pathology and Lab Medicine

  • 1601 Pathology and Lab Medicine: Basic

Authors

  • Lai, Chun-Fu, National Taiwan University Hospital, Taipei, Taiwan
  • Yang, Hsin-Chieh, Washington University in St. Louis, St. Louis, Missouri, United States
  • Song, Sheng-Kwei, Washington University in St. Louis, St. Louis, Missouri, United States
  • Lin, Tsen-Hsuan, Washington University in St. Louis, St. Louis, Missouri, United States
Background

Conventional MRI provides noninvasive assessment for disease progression but lacks the pathological specificity. Previously, we successfully developed diffusion basis spectrum imaging (DBSI) to assess coexisting axonal injury, demyelination, and inflammation. This study aims to test whether DBSI can noninvasively detect kidney pathologies after ischemia reperfusion injury (IRI).

Methods

Six 10-week-old female C57BL/6 mice received unilateral left kidney IRI for 30 minutes. Mice were euthanized and perfusion fixed with 4% PFA at 4-7 days after surgery. IRI and contralateral control (CTL) kidneys were harvested for ex vivo DBSI scans. The DBSI scan was performed on a 4.7-T scanner: TR = 1.5 s, TE = 33 ms, maximal b-value = 1,500 s/mm2, image slice thickness = 0.5 mm, in-plane resolution = 156 × 156 µm2. DBSI-assessed restricted (putative cellularity), hindered (putative cytotoxic edema) and fiber (putative interstitial fibrosis) were derived using a novel lab-developed software. H&E and Masson's trichrome (MT) stains were performed to validate DBSI findings.

Results

Representative H&E and MT images of IRI kidneys demonstrated obvious tubular injury, interstitial edema, inflammation (b) and fibrosis (c). The region of interest (ROI)s were defined on T2W anatomy image (D). The corresponding DBSI metrics suggested significant increased cellularity (higher restricted fraction, E) and reduced interstitial space (reduced hindered fraction, F) in outer medulla. Higher anisotropic fiber fraction in outer stripes of outer medulla of IRI group (G) indicated increased fibrosis at that region.

Conclusion

DBSI MRI could detect coexisting pathologies in kidneys after injury. It has potential to noninvasively follow kidney disease progress, monitor treatment efficacy, and translate to clinical application.

Funding

  • Other NIH Support