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Abstract: FR-PO210

Calcitriol Plays a Protective Role in Podocytes of Diabetic Nephropathy Rats by Regulating Autophagy

Session Information

Category: Diabetic Kidney Disease

  • 601 Diabetic Kidney Disease: Basic

Authors

  • Song, Zhixia, Center People's Hospital of Yichang, Three Gorges University, School of Medicine, Yichang, China
  • Xiao, Chao, Three Gorges University, School of Medicine, Yichang, China
  • Zhang, Shizhong, Three Gorges University, School of Medicine, Yichang, China
  • Jia, Xiaoli, Three Gorges University, School of Medicine, Yichang, China
  • Luo, Chunhua, Department of Clinical Laboratory, Center People''s Hospital of Yichang, Yichang, China
  • Shi, Lang, Three Gorges University, School of Medicine, Yichang, China
  • Xia, Rong, Center People's Hospital of Yichang, Three Gorges University, School of Medicine, Yichang, China
  • Yang, Lin, Center People's Hospital of Yichang, Three Gorges University, School of Medicine, Yichang, China
Background

Podocyte injury is the core link of the development of diabetic nephropathy(DN). The nature of podocyte proliferation determines the important role of intracellular degradation system in maintaining podocyte homeostasis. Evidences show that autophagy plays a critical role in the progression of podocyte disease. Previous research in our group has confirmed that calcitriol effectively reduce proteinuria and podocyte injury in DN rats. This study aimed to investigate whether calcitriol can reduce podocyte injury in DN rats by regulating autophagy.

Methods

DN model was established in male SD rats by intraperitoneal injection of streptozotocin(60mg/kg). The rats were subsequently given calcitriol or vehicle(0.1μg/kg/d) by gavage and then sacrificed at three time points(week8, 14, 18). Specimens were used for biochemical, histopathological and molecular biology tests.

Results

DN rats exhibited renal pathological damage accompanied by proteinuria. The expression of slit diaphragms proteins(low expression of nephrin, podocin, synaptopodin), VDR(low expression), autophagy-associated proteins(low expression of LC3B/A, Beclin1, overexpression of P62) were abnormal in DN rats. The number of autophagosomes in podocytes were significantly reduced. The fluorescence of LC3B and synaptopodin proteins were highly overlapping, the fluorescence intensity of both proteins in DN rats were significantly reduced. whereas calcitriol reversed these above changes.

Conclusion

Calcitriol reduces podocyte injury in DN rats by regulating autophagy, thereby reducing proteinuria.

Funding

  • Government Support - Non-U.S.