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Kidney Week

Abstract: FR-PO119

A Novel Circular RNA-has_circ_0114427 Regulates Inflammation via miR-494 in AKI

Session Information

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Tang, Wanxin, West China Hospital of Sichuan University, Chengdu, SiChuan, China
  • Cao, Yiling, West China Hospital of Sichuan University, Chengdu, SiChuan, China
  • Mi, Xuhua, West China Hospital of Sichuan University, Chengdu, SiChuan, China
  • Zhang, Dongmei, West China Hospital of Sichuan University, Chengdu, SiChuan, China
  • Wang, Zheng, West China Hospital of Sichuan University, Chengdu, SiChuan, China
  • Zuo, Yongdi, West China Hospital of Sichuan University, Chengdu, SiChuan, China
Background

Acute kidney injury (AKI) is a common serious syndrome characterized by a rapid decrease of glomerular filtration rate and the progressive increase of serum creatinine. CircRNAs are novel regulatory RNAs that recently became popular among researchers of various diseases. However, the expression profile and function of circRNAs in AKI remain largely unknown. CircRNAs act as competing endogenous RNAs (ceRNAs) to regulate transcription level by binding with microRNAs (miRNAs), as indicated by recent research.

Methods

In the current study, we established a cisplatin-induced AKI mice model and then extracted circRNAs from isolated renal tubular tissues for next-generation sequencing at different time points during AKI's early stage. By using bioinformatic analysis, we identified out a certain number of significant differentially expressed mmu-circRNAs in AKI. Furthermore, we validated the expression pattern and explored the function of the significant homologous circRNAs in HK2 cells.

Results

We successfully identified differentially expressed circRNAs related to AKI. By finding homologous genes between mouse and human, we identified a new circRNA, circ-0114427, in humans. Circ-0114427 expression was significantly up-regulated in different AKI cell models. Knockdown of circ-0114427 indicated that circ-0114427 bound to miR-494 as a miRNA sponge to regulate ATF3 expression and further affected the expression of downstream cytokine IL-6.

Conclusion

Elevated circ-0114427 may play an important role in anti-inflammation in the early stage of AKI. Our findings provide a novel insight into the regulatory mechanism of circRNAs in AKI and may become a new molecular target resource for early diagnosis and treatment strategies.

The workflow of our experiments