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Abstract: FR-PO379

Effect of Magnesium on the Processes of Inflammation and Oxidative Stress Associated with CKD

Session Information

  • CKD: Mechanisms - II
    November 08, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: CKD (Non-Dialysis)

  • 2103 CKD (Non-Dialysis): Mechanisms

Authors

  • Rodriguez Ortiz, Maria Encarnacion, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain
  • López baltanás, Rodrigo, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain
  • Diaz Tocados, Juan M., Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain
  • Canalejo, Antonio, University of Huelva, Huelva, Spain
  • Rodriguez, Mariano, Hospital Universitario Reina Sofia, Cordoba, Spain
  • Munoz-Castaneda, Juan R., Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain
  • Almaden peña, Yolanda, Lipid and Atherosclerosis Unit. Department of Internal Medicine/ IMIBIC/Reina Sofia University Hospital/University of Cordoba, Cordoba, Spain
Background

The development and progression of vascular calcifications (VC) is a prevalent complication in advanced stages of chronic kidney disease (CKD). Previous observations show that magnesium (Mg) may be beneficial in preventing the development of VC. Uremia is considered to be an inflammatory state, and both chronic inflammation and oxidative stress appear to have a causal effect in VC by directly affecting vascular smooth muscle cells (VSMC). The main goal of this work was to assess, through both in vivo and in vitro approaches, the effect of Mg on inflammation and oxidative stress associated with CKD.

Methods

In vitro studies were based on the culture of VSMC in the presence of high phosphorus (P), with or without Mg. In vivo experiments were performed in an experimental model of uremic rats feeding with high P diet and Mg dietary supplementation during 14 days. All experimental protocols were reviewed and approved by the Ethics Committee for Animal Research of the University of Cordoba (Cordoba, Spain) and adhered to the recommendations included in the Guide for Care and Use of Laboratory Animals (US Department of Health and Human Services, NIH) and European laws and regulations on protection of animals, under the advice of specialized personnel.

Results

VSMC incubated with high P exhibited an increase in pro-inflammatory mediators, the inflammatory cytokines and the levels of oxygen reactive species (ROS). The addition of Mg prevented the elevation in inflammatory markers.
Uremic rats receiving normal dietary Mg showed elevated levels of ICAM-1 and high oxidative stress By contrast, dietary Mg supplementation abolished all these processes.

Conclusion

Taken together, these results suggest the protective role of Mg in the generation of oxidative stress and inflammation in the context of renal disease.