Abstract: SA-PO248
Comparison Between a Novel Latex Immunoassay and LC-MS/MS for Hepcidin-25 Measurement
Session Information
- Anemia and Iron Metabolism: Clinical
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Anemia and Iron Metabolism
- 202 Anemia and Iron Metabolism: Clinical
Authors
- Kamei, Daigo, Tokyo Women's Medical University, Tokyo, Japan
- Nagano, Masashi, Nerima Sakuradai Clinic, Tokyo, Japan
- Hanafusa, Norio, Tokyo Women's Medical University, Tokyo, Japan
- Mineshima, Michio, Tokyo Women's Medical University, Tokyo, Japan
- Nitta, Kosaku, Tokyo Women's Medical University, Tokyo, Japan
- Tsuchiya, Ken, Tokyo Women's Medical University, Tokyo, Japan
Background
Hepcidin-25 is an iron regulatory factor in the in vivo evaluation of iron dynamics and plays an important role in determining the development and severity of anemia in patients with chronic kidney disease.
Although the golden standard of measurement of hepcidin-25 is the LC-MS/MS method, results cannot be obtained immediately at the clinical site. However, the latex immunoassay (LIA) can be performed using general clinical laboratory equipment, and the results obtained quickly.
Our aim was to measure hepcidin-25 by LIA and LC-MS/MS and compare the two methods.
Methods
Hepcidin-25 was measured by LIA and LC-MS/MS in 134 hemodialysis patients. We used a hepcidin-25 specific reagent (FUJIFILM Wako Pure Chemical Corporation) and the JCA-BM6050 automatic analyzer for LIA and the 4000 QTRAP LC-MS/MS system for LC-MS/MS. The results obtained by the two methods were compared by standard major axis regression.
Results
The standard major axis regression equation between the two methods was y = 0.995x + 0.5 (r = 0.998, p < 0.001). The correlation between the two methods was very strong and the measured values were almost identical.
Conclusion
The performance of LIA was equivalent to that of LC-MS/MS for hepcidin-25 measurement. LIA can be performed using general clinical examination apparatus and has a higher processing speed than LC-MS/MS. Therefore, measurement of hepcidin-25 by LIA may potentially be useful for routine laboratory testing.