Abstract: FR-PO965
High-Throughput Determination of Aminoglycoside Antimicrobials in Human Plasma by Hydrophilic Interaction Liquid Chromatography Mass Spectrometry
Session Information
- Pathology and Lab Medicine: Basic
November 08, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Pathology and Lab Medicine
- 1601 Pathology and Lab Medicine: Basic
Authors
- Omiya, Shinya, Division of Nephrology, Department of Medicine, Showa University Fujigaoka Hospital, Yokohama, Japan
- Lee, Xiao-Pen, Showa University Department of Legal medicine, Tokyo, Japan
- Kumazawa, Takeshi, Seirei Christopher University, Shizuoka-ken, Japan
- Kawata, Naoto, Division of Nephrology, Department of Medicine, Showa University Fujigaoka Hospital, Yokohama, Japan
- Miyazaki, Tomoaki, Division of Nephrology, Department of Medicine, Showa University Fujigaoka Hospital, Yokohama, Japan
- Inui, Kiyoko, Division of Nephrology, Department of Medicine, Showa University Fujigaoka Hospital, Yokohama, Japan
- Inoue, Yoshihiko, Division of Nephrology, Department of Medicine, Showa University Fujigaoka Hospital, Yokohama, Japan
- Yoshimura, Ashio, Shinyokohama Daiichi Clinic, Yokohama, Japan
- Koiwa, Fumihiko, Division of Nephrology, Department of Medicine, Showa University Fujigaoka Hospital, Yokohama, Japan
Background
Aminoglycoside antibiotics can cause acute kidney injury (AKI) due to tubular necrosis in a dose-dependent manner.Therefore, a qualitative and quantitative method for determining AG antibiotics in blood must be useful in prevention of AKI.
Methods
We developed a simple and rapid method for the analysis of six AG antibiotics, such as streptomycin, ribostamycin, kanamycin, amikacin, dibekacin, and arbekacin, in human plasma samples by hydrophilic interaction liquid chromatography (HILIC)-tandem mass spectrometry (MS/MS).Each sample was 50µl of plasma.
Results
All drugs showed base peaks due to [M+H]+ ions by HILIC-MS with positive ion electrospray ionization, and the product ions were produced from each [M+H]+ ion by HILIC-MS/MS. Quantitation was performed by selective reaction monitoring using each base peak of product ions of HILIC-MS/MS. Good peak shapes of the six drugs were achieved within an analysis time of 1.4 min. All drugs spiked into plasma showed recoveries of 23-77% and extraction efficiencies of 72-105%. The regression equations for the antibiotics showed excellent linearity with the limits of quantitation of 3.9-16 µg/ml. The intra- and inter-day relative standard deviations for all drugs were not greater than 19%. The accuracies of quantitation were 80-114%. Streptomycin and kanamycin in human plasma after intramuscular administration of the drugs could actually be determined.
Conclusion
A simple and rapid method was developed for the analysis of six AG antibiotics in human plasma by HILIC-MS/MS. This method seem to be useful for determining AG antibiotic residues in the edible tissues of livestock in addition to human plasma. therefore, it will be widely used in patients with kidney dysfunction who need administration of AG.