Abstract: TH-PO495
The Competing Endogenous RNA Network in Renal Aging
Session Information
- CKD: Mechanisms - I
November 07, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2103 CKD (Non-Dialysis): Mechanisms
Authors
- Li, Jie, First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi Xi'an, China
- Jiang, Hongli, First Affiliated Hospital of Medicine School, Xi'an Jiaotong University, Xi'an, China
Background
It is well known that aging is a continuous and gradual process that causes the physiological functions of all organ systems in the human body to gradually decline. The kidney, which is a metabolically active organ, is extremely susceptible to aging, but the mechanism of kidney aging is unclear. MicroRNAs (miRNAs) are a highly conserved small non-coding RNA of 18-25 nucleotides in length that inhibits protein expression at the post-transcriptional level or degrades target genes to regulate gene expression. Long-chain non-coding RNA (lncRNA) is a non-coding RNA consisting of 200 nucleotides. It is generally considered that they do not encode proteins, but are expressed in various forms at the RNA level. In ceRNA networks, lncRNAs bind to miRNAs via miRNA response elements (MREs), and miRNAs can also bind to the corresponding mRNA 3'UTR through specific binding sites, inhibiting the level of protein expressed.
Methods
Analyse the lncRNA expression profiles in different ages mouse using a microarray array. And predicted miRNAs and mRNAs that interact with lncRNAs. SA-β-gal staining and immunohistochemistry were operated for the detection of the p53, p16 expression in different ages. Then we use Masson and PAS staining to assess the degree of fibrosis in the kidney.
Results
Bioinformatics analysis results show that the expression of some lncRNAs decreased with age, and the corresponding miRNAs expression increased accordingly. With the increase of age, we detected that the expression of senescence-associated galactosidase gradually increased, the expression of p16 and p53 gradually increased, and the renal fibrosis gradually worsened.
Conclusion
With the increase of age, the expression of senescence-associated galactosidase gradually increased, the expression of p16 and p53 gradually increased, and the renal fibrosis gradually worsened. ceRNA network plays an important role in kidney aging.