Abstract: SA-PO692
Circulating Endothelial Microparticles and Correlation with MEST-C Scores in IgA Nephropathy
Session Information
- Pathology and Lab Medicine: Clinical
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Pathology and Lab Medicine
- 1602 Pathology and Lab Medicine: Clinical
Authors
- Agrawal, Vinita, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India
- Bharti, Niharika, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India
- Agarwal, Vikas, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India
- Prasad, Narayan, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India
Background
Renal endothelial injury or activation can lead to a cascade of pathways that contribute to loss of renal function and fibrosis. Recent reports suggest that endothelial injury may play a role in the pathophysiology of IgAN. Microparticles (MPs) are 0.1 to 1.0 µm membrane vesicles shed from the damaged or activated cell surface following injury. Microparticles derived from endothelial cells are called endothelial microparticles (EMPs) and play an important role in promoting endothelial dysfunction. They can act as biomarkers of disease state and progression. The aim of this study was to study the presence and quantify the levels of circulating MPs of endothelial origin in plasma from patients with IgAN and healthy controls.
Methods
25 biopsy-proven IgA nephropathy (Mean age=32.8±8.2 years) and 25 healthy controls (Mean age=30±7.6 years) were recruited in this study. Platelet-poor-plasma from citrated blood was isolated and centrifuged at 20,000g (90 min) at 4 degree C. EMPs were analyzed by Flow cytometry using EMP specific antibodies for antiCD31-FITC and antiCD146-PE. All quantification related to size and number was done by using cell count beads of known concentration. The levels of circulating endothelial MPs were correlated with renal biopsy features of the Oxford classification (MEST-C scores). The study was reviewed and approved by the Institutional Ethics Committee.
Results
There are significantly higher levels of total circulating MPs and EMPs in IgAN compared to healthy controls (p<0.05). EMPs levels were increased in patients with hypertension and correlated with presence of mesangial and endocapillary hypercellularity (p<0.05) on renal biopsy (Table 1).
Conclusion
IgAN shows evidence of significant endothelial injury/dysfunction. A non-invasive method of detection of levels of circulating EMPs can predict the severity of glomerular injury and may be a simple method useful for monitoring endothelial injury in IgAN.
Table 1. Correlation of Circulating Endothelial Microparticle (EMP) counts with MEST-C scores in IgAN
| MEST-C score on Renal Biopsy | MPs/μl plasma | Circulating EMPs/μl plasma (CD31+/CD146+) | P value |
| Mesangial Proliferation M0 M1 | 105±12 187±107 | 21±2.8 44±27 | 0.04 |
| Endocapillary Proliferation E0 E1 | 141±49 242±141 | 31±14 60±33 | 0.001 |
| Segmental sclerosis S0 S1 | 168±93.3 188±116 | 40±26 43±26 | 0.614 |
| Tubular Atrophy Absent Present | 126±34 156±89 | 37±13 44±29 | 0.433 |
| Crescent Absent Present | 164±82 200±140 | 39±26 45±27 | 0.512 |
Funding
- Government Support - Non-U.S.