Abstract: SA-PO041
Culturing of Murine Podocytes via a 3D Suspension Culture System Using the Microcarrier
Session Information
- Engineering-Based Approaches to Problems in Nephrology
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Bioengineering
- 300 Bioengineering
Authors
- Yu, Chih-Chuan, Kaohsiung Medical University, Kaohsiung, Taiwan
- Hwang, Daw-yang, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan
- Chang, Jer-Ming, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan
Background
Cell culture in two dimensions has been well established in the worldwide for the past decades. However, the culture of cells in two dimensions is unable to representative of real cell environments, lack of predictively to anatomy or physiology in vivo. Creating a third dimension for cell culture is clearly a better way of representing physiological relevant then 2D culture.
Methods
Murine poodcytes were determined that stirred suspension bioreactors utilizing Cytodex-3 microcarrier beads represent a viable platform for the differentiation of podocytes. 1 gram microcarrier beads were loaded, an inoculation ratio of 2 x 107 cells per 1 gram beads, and discontinuous agitation in a medium with 10% serum resulted in high cell attachment efficiencies.
Results
At the end of incubation, the expression levels of nephrin and synaptopodin were examined after various microcarriers beads culture time periods. Compared to static tissue culture dishes, a bioreactor-based bioprocess requires fewer handling steps, lower operating cost of culture consumable, less differentiation time needed when compared to 2D culture.
Conclusion
stirred suspension bioreactors incorporating microcarrier technology represent a viable and more efficient platform than tissue culture flasks for the generation of differentiated podocytes in culture.
Funding
- Government Support - Non-U.S.