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Kidney Week

Abstract: TH-PO905

Urinary L-FABP Reflects the Degree of Sarcopenia in a Diabetic Kidney Disease Model

Session Information

Category: Diabetic Kidney Disease

  • 601 Diabetic Kidney Disease: Basic

Authors

  • Tanabe, Jun, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
  • Ichikawa, Daisuke, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
  • Kimura, Kenjiro, Tokyo Takanawa Hospital, Tokyo, Japan
  • Shibagaki, Yugo, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
  • Ikemori, Atsuko, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan
Background

Diabetic kidney disease (DKD) is a high-risk factor for onset of sarcopenia which leads to increase in all-cause mortality in patients with type 2 diabetes. Although the sarcopenia in DKD should be focused just before a super-aging society, there had not been a useful biomarker for monitoring the sarcopenia. The aim of this study is to reveal the correlation between urinary L-type fatty acids binding protein (L-FABP) known as a tubular marker and sarcopenia using novel model of DKD with sarcopenia.

Methods

Male spontaneously diabetic torii (SDT) fatty rats (n =5) were used as an animal model of type 2 diabetes with sarcopenia. Age- and sex-matched Sprague–Dawley rats (SD) (n = 7) were used as controls. Urine samples were obtained from the rats at 8, 12, 16, 20, and 24 weeks of age and muscle strength was evaluated by forelimb grip test at 12, 16, 20, and 24 weeks of age. Their kidney, and soleus and extensor digitorum longus (EDL) muscles were obtained at 24 weeks of age.

Results

Urinary L-FABP increased and muscle strength decreased along with age in the SDT rats. Renal tissue damage and accumulation of renal oxidative protein were observed at 24 weeks of age of the SDT fatty rats. Muscle weight, and cross-sectional areas of both type I and type IIb muscle fibers were significantly decreased in the SDT fatty rats compared to the SD rats. The muscle atrophy in the SDT fatty rats was induced due to decreased phosphorylation of S6K1 and increased expression of E3 ubiquitin ligases, atrogin-1 and murf-1. The levels of urinary L-FABP and the degree of renal tissue damage were significantly correlated with muscle weigh, diameter of muscle fibers and muscle strength.

Conclusion

Urinary L-FABP increased along with the progression of sarcopenia and reflected the degree of sarcopenia in DKD. In clinical practice, urinary L-FABP may be useful for monitoring the sarcopenia as well as DKD in the type 2 diabetic patients.