Abstract: SA-PO758
Variations in Gut Microbiota May Correlate with Lipid Metabolism in UMOD Knockout Rats
Session Information
- CKD: Mechanisms - III
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2103 CKD (Non-Dialysis): Mechanisms
Author
- Wang, Na, Renal Division, Peking University First Hospital Institute of Nephrology, Peking University, BeiJing, China
Background
Tamm-Horsfall protein (THP,uromodulin,UMOD) is an important protective factor during kidney injury. Although it was mainly expressed in the thick ascending limb, uromodulin still can be detected in the serum and in other tissue cells. Study has revealed a crucial role of uromodulin on the M-cell surface for the uptake of SlpA-positive lactic acid bacteria into M cells, possibly leading to subsequent delivery of the bacteria to dendritic cells closely associated with M cells for immunomodulation. In this study we investigated the change of gut microbiome in UMOD ablation rats.
Methods
10wild type SD male rats and 10 UMOD knockout SD rats (THP-/-) were housed under controlled environment for10 weeks. Same water and diet were provided. All of the rats were anesthetized to collect fecal samples from large intestine directly. The bacterial composition was analyzed based on 16S ribosomal DNA pyrosequencing. Bioinformatics tools, including sequence alignment, abundance, and taxonomic diversity, were used in microbiome data and its products analyses.
Results
There was no difference in mean weight between two groups. The serum triglyceride (TG) decreased significantly in THP-/-rats (0.632&1.473mmol/L, p<0.05). The microbial richness and diversity in composition were different in THP-/- rats compared with WT (PCoA analysis, p=0.036). At the phylum level, there were obvious reductions in Elusimicrobia and Actinobacteria in THP-/- rats (p<0.05). At the genus level, five genera were obviously increased in THP-/- rats(p<0.05), including Helicobacter, Lactobacillus, Roseburia, Clostridium XI and Phascolarctobacterium. While four genera,including Alloprevotella, Elusimicrobium ,Intestinimonas and Ruminococcus decreased (p<0.05). Among those changed genera, Ruminococcus and Elusimicrobium presented positive correlation with TG, while Phascolarctobacterium, Lactobacillus and Alloprevotella were negative correlated with TG (P<0.05).Obvious variations were identified in 33 microbial metabolites with the change of microbiota. 22 out of 33 microbial metabolite products were fatty acid.
Conclusion
UMOD ablation led to significant variations in composition of gut microbiota in SD rats. The change of gut microbiota correlated with serum TG, suggested other pathways may be involved the abnormal lipid metabolism in chronic kidney disease