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Abstract: SA-PO752

Dietary Phosphate Disturbs of Gut Microbiome In Mice

Session Information

  • CKD: Mechanisms - III
    November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
    Abstract Time: 10:00 AM - 12:00 PM

Category: CKD (Non-Dialysis)

  • 2103 CKD (Non-Dialysis): Mechanisms

Authors

  • Oda, Naoko, Tokushima University, Tokushima, Japan
  • Sugihara, Kohei, Tokushima University Graduate School, Tokushima, Japan
  • Ikeda, Miho, Tokushima University, Tokushima, Japan
  • Higashimura, Yuho, Tokushima University, Tokushima, Japan
  • Uebanso, Takashi, Institute of Medical Nutrition, Tokushima Uiverisity, Medical School, Tokushima City, Japan
  • Ohminami, Hirokazu, Tokushima University, Tokushima, Japan
  • Ohnishi, Kohta, Tokushima University, Tokushima, Japan
  • Masuda, Masashi, Tokushima University, Tokushima, Japan
  • Yamanaka-Okumura, Hisami, Tokushima University, Tokushima, Japan
  • Taketani, Yutaka, Tokushima University, Tokushima, Japan
Background

Disorder of phosphate metabolism is a common pathological condition in chronic kidney disease (CKD) patients. Excessive intake of dietary phosphate deteliorates CKD and various complications including cardiovascular disease. Recent reports have demonstrated that gut microbiome disturbance is associated with both the etiology and progression of CKD. However, the relationship between dietary phosphate and gut microbiome remains unknown. Here, we examined the effects of excessive intake of phosphate on gut microbiome.

Methods

Five-week-old male C57BL/6J mice were fed either control diet (0.4% phosphate; CP) or high phosphate diet (1.2% phosphate; HP) for eight weeks. Stool samples were collected at eight weeks. After amplifying the V3-V4 region of 16S ribosome RNA by PCR, analysis of the gut microbiota was carried out using MiSeq next generation sequencer (NGS).

Results

Compared with CP diet group, HP diet group significantly decreased in body weight and epididymal fat weight, plasma calcium level, and increased in urinary phosphate excretion. In analysis of gut microbiota, HP diet group increased in Firmicutes phylum and decreased in Bacteroidetes phylum by PCR and NGS analysis. In particular, significant increase in Erysipelotrichaceae genus and decrease in Clostridia genus were observed in HP diet group, and NGS analysis showed the decrease in bacterial diversity in HP diet group. In addition, HP diet group decreased colonic tight junction marker mRNA levels.

Conclusion

These results suggest that the excessive intake of dietary phosphate disturbs gut microbiota and decreased in bacterial diversity. Furthermore, it may affect the intestinal barrier function. Such a disturbance may be related to progression of CKD. Adequate management of dietary phosphate would be required to keep healthy environment in gut.

Funding

  • Government Support - Non-U.S.