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Abstract: SA-PO995

A Study on Prevalence of Hepatitis C Virus Infection and Its Genotype Distribution Among Chronic Renal Failure Patients on Maintenance Hemodialysis: A Single-Center Experience

Session Information

Category: Dialysis

  • 701 Dialysis: Hemodialysis and Frequent Dialysis

Authors

  • Patel, Minaxi, Institute of Kidney Diseases and Research Center - Institute of Transplantation Sciences, Ahmedabad, India
  • Patel, Himanshu V., Institute of Kidney Disease and Research Centre, Ahmedabad, India
  • Patel, Ansy Himanshu, BJ Medical College, Ahmedabad, India
Background

Hepatitis c virus (HCV) infection is a very common infection in chronic renal failure (CRF) patients on maintenance hemodialysis (HD).Genotype detection is crucial for management of chronic hepatitis c patients, prediction of prognosis, epidemiological study and also for vaccine preparation. Based on the sequence divergence, till date HCV strains are divided into 7 main genotypes and multiple subtypes (67 confirmed, 20 provisional).

Methods

The aim of this study was to find out single centre prevalence and distribution of HCV genotypes in CRF patients on maintenance haemodialysis (MHD).
Genotyping was performed by nested reverse transcriptase PCR. Isolation of HCV RNA, reverse transcription and nucleic acid amplification of 5’ UR was carried out. Biotinylated oligonucleotide primers were used to generate amplified product and reversely hybridized to type-specific probes on nitro-cellulose strips. Conjugate and substrate were added post-hybridization to observe the generated bands which were then matched with control bands. The genotypes studied were 1a to1c, 2a to 2d, 3a to 3f, 4a to 4k, 5a and 6a.

Results

Out of 2550, 210 patients (12.14%) (Male: 166, Females: 44) were HCV positive. Genotype 1 was found in 179 (85.2%) and genotype 3 in 31(14.8%) patients. Amongst genotype 1, subtype 1a, 1b, 1ba and undetermined comprised 71.4%, 24.6%, 0.6% and 3.4% respectively. Amongst genotype 3, subtypes 3a, 3b and undetermined comprised 70.9%, 9.7% and 19.4% respectively. No other genotypes were found.

Conclusion

HCV infection was found in 12.14 % CRF patients on MHD with genotype 1 (85.2%) being predominant followed by genotype 3 (14.8%) in our study.