Abstract: SA-PO437
Extracellular Vesicles from Human Bone Marrow Mesenchymal Stem Cells Repair Organ Damages Caused by Cadmium Poisoning in a Medaka Model
Session Information
- Development and Regenerative Medicine
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Development, Stem Cells, and Regenerative Medicine
- 500 Development, Stem Cells, and Regenerative Medicine
Author
- Obara, Tomoko, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States
Background
Treatment modalities for kidney disease caused by long-term exposure to heavy metals, such as cadmium (Cd), are limited. Often, chronic, long-term environmental exposure to heavy metal is not recognized in the early stages; therefore, chelation therapy is not an effective option. Extracellular vesicles (EVs) derived from stem cells have been demonstrated to reduce disease pathology in both acute and chronic kidney disease models.
Methods
To test the ability of EVs derived from human bone marrow mesenchymal stem cells (hBM-MSCs) to treat Cd damage, we generated a Cd-exposed medaka model that we treated with EVs.
Results
This model develops heavy metal-induced cell damage of various organs and tissues, and shows decreased survival. Intravenous injection of highly purified EVs from hBM-MSCs repaired the damage to kidney proximal tubules apical and basolateral membranes, and mitochondria, glomerulus podocytes, repaired bone deformation caused by Cd, and enhanced survival.
Conclusion
Our system serves as a model with which to study age- and sex-dependent cell injuries of organs caused by various agents and diseases. The effects of EVs on the tissue repair process, as shown in our Cd-exposed medaka model, may open new broad avenues for interventional strategies.
Funding
- Private Foundation Support