Abstract: FR-PO725
Development and Implantation of Novel Morphometric 3D Capsule Device to Constrain Structural Change of Polycystic Kidney: A Feasibility Study in a Rat Model
Session Information
- Cystic Kidney Diseases: Clinical/Translational
November 08, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Genetic Diseases of the Kidneys
- 1001 Genetic Diseases of the Kidneys: Cystic
Authors
- Bae, Kyongtae Ty, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
- Kumamoto, Kanako, Education and Research Center of Animal Models for Human Diseases, Fujita Health University, Toyoake, Aichi, Japan
- Yoshimura, Aya, Fujita Health University, Toyoake, Aichi, Japan
- Kugita, Masanori, Fujita Health University, Toyoake, Aichi, Japan
- Horie, Shigeo, Juntendo University, Tokyo, Japan
- Yamaguchi, Tamio, Department of Clinical Nutrition, Suzuka University of Medical Science, Suzuka, Mie, Japan
- Bae, Junu, Ohio State University, Columbus, Ohio, United States
- Nagao, Shizuko, Fujita Health University, Toyoake, Aichi, Japan
Background
Polycystic kidney disease is characterized by progressive enlargement of kidneys as a consequence of uninhibited formation and expansion of numerous kidney cysts. Current therapeutic options for polycystic kidney disease are limited in their effectiveness at halting disease progression. The objectives of the study were (1) to develop and implant a computed tomography (CT) image-derived morphometric 3D capsule device to encase a kidney and (2) to demonstrate experimental outcome of the device to constrain structural change of polycystic kidney in a rodent model.
Methods
Kidney capsule devices were designed from CT images of wild-type and Cy/+ rats. Capsule devices were surgically implanted on kidneys in six surgical sessions over a period of 14 months in 7 wild-type rats 6.5-8 weeks of age (3 sham operation, 2 right, 2 left) and 6 Cy/+ rats 6.5 weeks of age (2 sham, 3 left, 1 bilateral). After operation, rats were followed for 5.4-12.4 weeks to grow and sacrificed to retrieve kidneys. During the follow-up, serum creatinine was measured. Retrieved kidneys were weighed. Histological analysis including cystic area measurement and immunohistochemistry was performed.
Results
Morphometric capsule devices were configured and developed by image processing technique and produced using 3D printing technique. Encapsulated Cy/+ kidneys (n=5; mean weight 3.64g) were consistently smaller in size (by 21-36%; p<0.001) than unencapsulated Cy/+ kidneys (n=7; mean weight 5.52g). Encapsulated Cy/+ kidneys (mean %cyst area: 29.4%) showed smaller histologic cystic area (by 28-58%; p<0.001) than unencapsulated Cy/+ kidneys (mean %cyst area 48.6%). Cell proliferation and macrophages were also markedly reduced in encapsulated Cy/+ kidneys, compared to unencapsulated Cy/+ kidneys.
Conclusion
We developed a CT image-derived morphometric 3D capsule device to encase a kidney and demonstrated that the device constrained structural change of polycystic kidney in a rodent model as a feasibility study toward a novel potential therapeutic avenue for halting progression of polycystic kidney disease.