ASN's Mission

ASN leads the fight to prevent, treat, and cure kidney diseases throughout the world by educating health professionals and scientists, advancing research and innovation, communicating new knowledge, and advocating for the highest quality care for patients.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005


The Latest on Twitter

Kidney Week

Abstract: FR-PO1096

The Ribonuclease 6 Antimicrobial Peptide Limits Bacterial Burden During Experimental Pyelonephritis

Session Information

Category: Pediatric Nephrology

  • 1700 Pediatric Nephrology


  • Cortado, Hanna H., Nationwide Children's Hospital, Columbus, Ohio, United States
  • Jackson, Ashley R., Nationwide Children's Hospital, Columbus, Ohio, United States
  • Li, Birong, Nationwide Children's Hospital, Columbus, Ohio, United States
  • Ching, Christina B., Nationwide Children''s Hospital, Columbus, Ohio, United States
  • Spencer, John David, The Research Institute at Nationwide Children's, Columbus, Ohio, United States
  • Becknell, Brian, Nationwide Children's Hospital, Columbus, Ohio, United States

Group or Team Name

  • Nephrology and Urology Research Affinity Group

Ribonuclease 6 (RNase 6) is an evolutionarily-conserved antimicrobial peptide that kills uropathogenic bacteria at low micromolar concentrations in vitro. Here, we investigated the hypothesis that RNase 6 limits urinary tract colonization by uropathogenic Escherichia coli (UPEC) in vivo.


We generated mice with a Rnase6EGFP knock-in allele on a C57BL/6J genetic background. We identified cellular sources of RNase6 based on flow cytometry, epifluorescence, and immunofluorescence microscopy. We transurethrally inoculated Rnase6EGFP/EGFP and control female mice with UPEC strain CFT073 and enumerated bacterial burden in urinary tract tissues by homogenization and serial plating.


Flow cytometry in Rnase6EGFP/+ mice identified EGFP expression by circulating Ly6Chi monocytes which were recruited to the infected bladder by 6 hours post inoculation (hpi). In addition, EGFP was expressed by two discrete resident macrophage populations within the kidney. We confirmed Rnase6 deletion in Rnase6EGFP/EGFP mice, which displayed normal urinary tract development, fertility, and hematopoiesis. Rnase6 deficiency led to increased renal and ureteral UPEC burden at 6 and 12 hpi, compared to control mice.


In the infected urinary tract, RNase6 is primarily expressed by resident macrophages and recruited monocytes. We have demonstrated a critical role for RNase 6 in UPEC clearance from the upper urinary tract during ascending UTI in vivo.


  • Other NIH Support