Kidney Week

Abstract: SA-PO783

Short Time of Resistance Training Attenuates the Renal AKT Pathway; Improves Physical Capacity, Renal Function, and Glomerulosclerosis; and Decreases Mortality Rate in Rats with CKD

Session Information

• CKD: Mechanisms - III
  November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
  Abstract Time: 10:00 AM - 12:00 PM

Category: CKD (Non-Dialysis)

• 2103 CKD (Non-Dialysis): Mechanisms

Authors

• Luiz, Rafael, UNIFESP, São Paulo, Brazil

Rafael Luiz,

• Saud, Alexandre, UNIFESP, São Paulo, Brazil

Alexandre Saud,

• Gloria, Maria A., UNIFESP, São Paulo, Brazil

Maria A. Gloria,

• Rampaso, Rodolfo Rosseto, UNIFESP, São Paulo, Brazil

Rodolfo Rosseto Rampaso,

• Chiloff, Daniela Mendes, Escola Paulista de Medicina - UNIFESP, São Paulo, Brazil

Daniela Mendes Chiloff,

• Martinez, Beatriz Pedretti, Centro Universitário das Américas, São Paulo, Brazil

Beatriz Pedretti Martinez,

• Zavadzki, Giovanna Melanie, UNIFESP, São Paulo, Brazil

Giovanna Melanie Zavadzki,

• Ramires, Marina Larissa vettorello, FICSAE, Sao Paulo, Brazil

Marina Larissa vettorello Ramires,

• Goes, Miguel Angelo, Federal University of Sao Paulo, Sao Paulo, SÃO PAULO, Brazil

Miguel Angelo Goes,

Background

The aim of this study was to evaluate if 4 weeks of resistance training (RT) attenuates renal AKT Pathway, improves physical capacity (strength gain and VO₂ peak), renal function, glomerulosclerosis and mortality in rats with chronic kidney disease (CKD) by nephrectomy 5/6 (Nx5/6).

Methods

Adult Wistar rats were divided in four groups (n=8): Sedentary (S) Exercise (E), Nx 5/6 + Sedentary (NS), Nx 5/6 + Exercise (NE). We evaluated (by multiplex) renal AKT Pathway (IGF1R, TSC2, AKT, Mtor and PS706SK), creatinine clearance (CrCl), proteinuria (uProt), blood urea nitrogen (BUN), glomerulosclerosis, mean arterial pressure (MAP) as well mortality rate. Exercise periods were as follows: 6 to 12 climbs/day, 5 days a week, during 4 weeks, 40 to 60% of maximal load test (MLT). The physical capacity was performed with maximal load test (MLT), ergoespirometry test (Vo₂ peak) and maximal exercise test (MEtest).

Results

The Renal AKT Pathway was increased in NS vs all group in all protein analyzed (IGF1R, TSC2, AKT, Mtor and PS706SK), CrCl was improve in NE (43%) vs NS group, (p<0.05). Proteinuria was different in NS and NE vs S and E groups but not in NS vs NE. BUN was higher in NS and NE vs S and E. Glomerulosclerosis was different in NS vs NE (p<0.05). The MAP was lower in NE vs NS group (p<0.05). Physical Capacity (MLT, VO₂ peak and Mtest) was increased in NE vs NS. A higher mortality rate was observed in NS (30%). Results suggested that the 4 weeks of RT minimize the impact of 5/6Nx IN renal AKT pathway by increase in physical capacity (MLT, VO₂ peak and Mtest), reduce the impact on CrCl (43%) and improve in glomerulosclerosis (44%).

Conclusion

These parameters indicate that exercise could have a protective effect, especially under this experimental protocol. Thus, this study suggests that the exercise plays a preventive role in mortality and could be an additional strategy to be employed in CKD.