Abstract: FR-PO487
A High Magnesium Concentration in Citrate Dialysate Prevents Oxidative Stress and Damage in Human Monocytes
Session Information
- Hemodialysis and Frequent Dialysis - IV
November 08, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Dialysis
- 701 Dialysis: Hemodialysis and Frequent Dialysis
Authors
- Vida, Carmen, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain
- Alique, Matilde, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain
- De Sequera, Patricia, University Hospital Infanta Leonor, Madrid, Spain
- Carracedo, Julia, Complutense University, Madrid, Spain
- Bodega, Guillermo, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain
- Ramirez, Rafael, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain
Background
The use of dialysis fluids (DF) during haemodialysis has been associated with an increased oxidative stress and reduced serum levels of magnesium (Mg), contributing to inflammation and immune system disorders. Since it has been demonstrated the role of Mg in modulating immune function and reducing oxidative stress, in this study we have characterized whether higher Mg concentrations in DF could protect from oxidative-inflammatory stress in immunocompetent cells.
Methods
The effect of citrate (CDF, 1 mmol/L) or acetate (ADF, 3 mmol/L) dialysates with 0.5 mmol/l Mg (routinely used) or with higher Mg concentrations (1, 1.25 and 2 mmol/L) were assessed in human monocyte culture (THP-1). The levels of reactive oxygen species (ROS), malondialdehyde (MDA) and reduced (GSH) and oxidized (GSSG) glutathione were quantified under basal and/or inflammatory conditions (stimulation with lipopolysaccharide, LPS, 1 μg/ml).
Results
In monocytes, 0.5 mmol/L Mg CDF produced lower basal ROS production in relation to ADF (p<0.05). Moreover, the increase of Mg in CDF resulted in a significant reduction of ROS production under basal and inflammatory conditions, which was extremely marked in 2 mmol/L Mg (p<0.001). These effects were not observed in ADF. Interestingly, in a dose-dependent manner, high doses of Mg in CDF reduced the oxidative stress observed in monocytes under basal conditions. In fact, 2 mmol/L Mg significantly decreased the levels of GSH, GSSG and MDA and the GSSG/GSH ratio in relation with 0.5 mmol/L Mg.
Conclusion
The CDF produces a lower ROS production compared to ADF. Increasing the concentration of Mg in the DF, especially in CDF, could have a positive and protective effect reducing oxidative stress and damage in immune cells.