ASN's Mission

ASN leads the fight to prevent, treat, and cure kidney diseases throughout the world by educating health professionals and scientists, advancing research and innovation, communicating new knowledge, and advocating for the highest quality care for patients.

learn more

Contact ASN

1401 H St, NW, Ste 900, Washington, DC 20005

email@asn-online.org

202-640-4660

The Latest on Twitter

Kidney Week

Abstract: FR-PO487

A High Magnesium Concentration in Citrate Dialysate Prevents Oxidative Stress and Damage in Human Monocytes

Session Information

Category: Dialysis

  • 701 Dialysis: Hemodialysis and Frequent Dialysis

Authors

  • Vida, Carmen, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain
  • Alique, Matilde, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain
  • De Sequera, Patricia, University Hospital Infanta Leonor, Madrid, Spain
  • Carracedo, Julia, Complutense University, Madrid, Spain
  • Bodega, Guillermo, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain
  • Ramirez, Rafael, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain
Background

The use of dialysis fluids (DF) during haemodialysis has been associated with an increased oxidative stress and reduced serum levels of magnesium (Mg), contributing to inflammation and immune system disorders. Since it has been demonstrated the role of Mg in modulating immune function and reducing oxidative stress, in this study we have characterized whether higher Mg concentrations in DF could protect from oxidative-inflammatory stress in immunocompetent cells.

Methods

The effect of citrate (CDF, 1 mmol/L) or acetate (ADF, 3 mmol/L) dialysates with 0.5 mmol/l Mg (routinely used) or with higher Mg concentrations (1, 1.25 and 2 mmol/L) were assessed in human monocyte culture (THP-1). The levels of reactive oxygen species (ROS), malondialdehyde (MDA) and reduced (GSH) and oxidized (GSSG) glutathione were quantified under basal and/or inflammatory conditions (stimulation with lipopolysaccharide, LPS, 1 μg/ml).

Results

In monocytes, 0.5 mmol/L Mg CDF produced lower basal ROS production in relation to ADF (p<0.05). Moreover, the increase of Mg in CDF resulted in a significant reduction of ROS production under basal and inflammatory conditions, which was extremely marked in 2 mmol/L Mg (p<0.001). These effects were not observed in ADF. Interestingly, in a dose-dependent manner, high doses of Mg in CDF reduced the oxidative stress observed in monocytes under basal conditions. In fact, 2 mmol/L Mg significantly decreased the levels of GSH, GSSG and MDA and the GSSG/GSH ratio in relation with 0.5 mmol/L Mg.

Conclusion

The CDF produces a lower ROS production compared to ADF. Increasing the concentration of Mg in the DF, especially in CDF, could have a positive and protective effect reducing oxidative stress and damage in immune cells.