Abstract: TH-PO566
Matting Calcium Crystals by Melamine Improves Stabilization and Prevents Dissolution
Session Information
- Bone and Mineral Metabolism: Basic
November 07, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Bone and Mineral Metabolism
- 401 Bone and Mineral Metabolism: Basic
Authors
- Awuah boadi, Eugenia, Institute for Clinical Research, Washington, District of Columbia, United States
- Bandyopadhyay, Bidhan, Institute for Clinical Research, Washington, District of Columbia, United States
Background
Kidney stone recurrence has been shown to be as high as 50% within 10 years of stone elimination with renal stone recurrence rate among individuals who have received some form of intervention being as high as 40%. Recent studies have implicated melamine, a nitrogen-rich crystalline compound used in making plastics in nephrotoxicity. Our previous findings show that melamine induces calcium crystal formation and growth in a concentration dependent manner, however melamine’s impact on recurrences remain unclear. We therefore investigated whether melamine's role in crystal stabilization/retention could be contributing to the increased rates of kidney stone recurrence.
Methods
To examine the role of melamine, oven dried preformed CaOx and CaP crystals incubated with melamine were analyzed under an SEM/EDS microscope; morphological and elemental composition of samples were collected and analyzed. Time dependent dissolution and stabilization studies were performed using Alizarin red pH 4.3 and 6.8 to identify CaOx, CaP respectively remaining in solution. Dissolution experiments were conducted in the presence of known crystal inhibitors.
Results
We show here that the presence of melamine increases crystal retention/stability even with the added presence of an inhibitor. Again ammeline, a similar triazine compound does not induce crystal growth. Similarly, our SEM/EDS analysis showed that melamine in the presence of calcium crystals acts as a nucleation site allowing for crystal deposition and ultimately crystal growth.
Conclusion
Together, our results highlights the mechanism utilized by melamine in calcium crystal growth as well as the pathological stabilization and retention of these crystals in the presence of known inhibitors of crystallization such as citrates, commonly used to alleviate the calcium stone conditions.
Funding
- NIDDK Support