Abstract: FR-PO762
Modeling Vascular Diseases Using Organ-Specific Endothelial Cells
Session Information
- Development and Organoid Models
November 08, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Development, Stem Cells, and Regenerative Medicine
- 500 Development, Stem Cells, and Regenerative Medicine
Authors
- Zhang, Tian, Nanyang Technological University, Singapore, Singapore
- Li, Pin, Nanyang Technological University, Singapore, Singapore
- Low, Jian hui, Lee Kong Chian School of Medicine, Singapore, Singapore
- Zhou, Bingrui, Lee Kong Chian School of Medicine, Singapore, Singapore
- Xia, Yun, Nanyang Technological University, Singapore, Singapore
Background
The establishment of vascular network initiates with the specification of endothelial cells and the formation of primitive vascular network. While primitive vascular network supplies nutrients to the developing embryo, postnatal vascular system mainly originates from aorta-gonad-mesonephros (AGM) that eventually gives rise to both endothelial and hematopoietic lineages. During development, homogeneous endothelial cells further differentiate to acquire organ-specific identities to accomplish diverse functions of different organs. Endothelial cells may also be derived from multipotent progenitor cells within certain mesodermal organs. Recent study has shown that renal progenitors could at least partially contribute to renal vasculature. We established a kidney organoid differentiation platform that can generate renal-specific endothelial cells from human pluripotent stem cells. This differentiation platform enables us to study vascular development and diseases in an organ-specific manner.
Methods
Kidney Organoid Differentiation
Single Cell RNA Sequencing
Disease Modelling
Results
We established a kidney organoid differentiation platform that can generate renal-specific endothelial cells from human pluripotent stem cells.
Conclusion
Recent study has shown that renal progenitors could at least partially contribute to renal vasculature. We established a kidney organoid differentiation platform that can generate renal-specific endothelial cells from human pluripotent stem cells. This differentiation platform enables us to study vascular development and diseases in an organ-specific manner.
Funding
- Government Support - Non-U.S.