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Abstract: TH-PO426

MicroRNA Profiling of Urinary Exosomes to Identify Appropriate Housekeeping Genes and Early Biomarkers of CKD in Humans

Session Information

Category: CKD (Non-Dialysis)

  • 2101 CKD (Non-Dialysis): Epidemiology, Risk Factors, and Prevention

Authors

  • Tiwari, Swasti, Sanjay Gandhi PGI, Lucknow, India
  • Kumari, Manju, Sanjay Gandhi PGI, Lucknow, India
  • Rai, Bhuvnesh, SGPGIMS, Lucknow, India
Background

In the last decade or so the CKD mortality increased by almost 30%, with diabetic kidney disease increased by 40% throughout the globe. Regular screening for kidney disease onset can lessen this burden. The potential of urinary exosome (UE) as a non-invasive source for kidney disease biomarkers has gained enormous research attention. However, the lack of optimal endogenous control for normalizing gene-expression in UE limits its translation into clinical practice.

Methods

Using microarray, we compared the microRNA profile of UE from early kidney disease subjects [with and without diabetes, serum creatinine < 2.0 mg/dl) with matched healthy controls. taqman-based RT-PCR was done for validation

Results

Around fifteen hsa-miRs were found constitutively expressed across the three groups. Out of these, four abundant miRs were validated by TaqMan-based RT-PCR (n=10-20/group). Also, we found twenty-seven differentially expressed miRs in the UE among the three groups. Pathway analysis revealed VEGF signaling, focal adhesion, and cytokine-cytokine receptor interaction pathways as major targets of the differentially expressed miRs. Among the the differentially expressed miRs, miR-200c-3p, let-7b-3p, miR-6812 and miR-320 were validated by TaqMan-based RT-PCR (n=10-20/group). Also, the target gene prediction of ten novel miRs, identified in the UE, was done using the bioinformatic approach.

Conclusion

In conclusion, we have identified miRs in UE with the potential to serve as 1) kidney disease biomarkers and, 2) endogenous controls, for normalizing miRNA expression in urinary exosomes in kidney disease subjects. Funded by ICMR

Funding

  • Government Support - Non-U.S.