Abstract: SA-PO347
Hypertension Is Associated with Podocyte Hypertrophic Stress and Detachment Among a Healthy Living Donor Cohort
Session Information
- Hypertension and CVD: Mechanisms
November 09, 2019 | Location: Exhibit Hall, Walter E. Washington Convention Center
Abstract Time: 10:00 AM - 12:00 PM
Category: Hypertension and CVD
- 1403 Hypertension and CVD: Mechanisms
Authors
- Naik, Abhijit S., University of Michigan, Ann Arbor, Michigan, United States
- Le, Dustin, University of Michigan, Ann Arbor, Michigan, United States
- Samaniego-Picota, Milagros D., Henry Ford Health Services, Detroit, Michigan, United States
- Cibrik, Diane Marie, University of Kansas Hospital, Parkville, Missouri, United States
- Wang, Su Qing, University of Michigan, Ann Arbor, Michigan, United States
- Chowdhury, Mahboob A., University of Michigan, Ann Arbor, Michigan, United States
- Wiggins, Roger C., University of Michigan, Ann Arbor, Michigan, United States
Background
HTN is a major cause of ESKD. Kidney donors are a highly selected cohort with normal renal function.Podocyte depletion is a major process by which kidney disease progression occurs. Therefore we tested the hypothesis that Mean Arterial Pressure (MAP) would be related to rate of podocyte detachment.
Methods
87 living donors that eventually donated were utilized. Two podocyte markers (podocin,nephrin) and a distal tubular/collecting duct marker (aquaporin2) were measured from urine pellet in spot samples normalized to creatinine. UPod:CR is a marker of podocyte detachment, Podocin to Nephrin ratio (UPod:Neph) of podocyte hypertrophic stress and Podocin to Aquaporin ratio (UPod:Aqp2) to understand relation of glomerular to tubular injury. Linear regression was adjusted for donor age, BMI, eGFR before donation.
Results
No donors were on antiHTN therapy.Mean SBP was 124±13, DBP was 73±10. See table and figure.
Conclusion
MAP is linearly related to podocyte detachment, hypertrophic stress and preferential glomerular injury even among healthy controls cleared for donation.
| UPod:CR (Podocyte Detachment) (Log Transformed) | Coef. | Std. Err. | P value | LCL | UCL |
| MAP (every 10 mm Hg) | 0.63 | 0.32 | 0.055 | -0.02 | 1.28 |
| eGFR (per 10 ml/min) | 0.05 | 0.21 | 0.83 | -0.38 | 0.47 |
| Donor Age (every 10 years) | 0.13 | 0.28 | 0.64 | -0.21 | 0.03 |
| BMI (Kg/m2) | -0.09 | 0.06 | 0.15 | -0.21 | 0.03 |
| UPod:Neph (Podocyte Hypertrophic Stress) (Log Transformed) | |||||
| MAP (every 10 mm Hg) | 0.82 | 0.33 | 0.01 | 0.17 | 1.48 |
| eGFR (per 10 ml/min) | -0.79 | 0.21 | 0.71 | -0.50 | 0.35 |
| Donor Age (every 10 years) | 0.03 | 0.29 | 0.91 | -0.5 | 0.6 |
| BMI (Kg/m2) | -0.09 | 0.06 | 0.16 | -0.22 | 0.04 |
| UPod:Aqp (Glomerular vs. Tubular Injury) (Log Transformed) | |||||
| MAP (every 10 mm Hg) | 1.14 | 0.33 | 0.001 | 0.49 | 1.79 |
| eGFR (per 10 ml/min) | -0.14 | 0.21 | 0.50 | -0.57 | 0.28 |
| Donor Age (every 10 years) | -0.21 | 0.28 | 0.46 | -0.78 | 0.35 |
| BMI (kg/m2) | -0.07 | 0.06 | 0.27 | -0.19 | 0.05 |
Funding
- NIDDK Support