Abstract: PO0631
Study on the Mechanism of Microinflammation with Uremia Caused by Lactobacillus Activation of Intestinal Macrophages by Mincle
Session Information
- CKD Mechanisms - 2
October 22, 2020 | Location: On-Demand
Abstract Time: 10:00 AM - 12:00 PM
Category: CKD (Non-Dialysis)
- 2103 CKD (Non-Dialysis): Mechanisms
Authors
- Sun, Lingshuang, First Affiliated Hospital of Medicine School, Xi’an Jiaotong University,, Xi'an, Shaanxi, China
- Jiang, Hongli, First Affiliated Hospital of Medicine School, Xi’an Jiaotong University,, Xi'an, Shaanxi, China
Background
My previous studies have found that Intestinal macrophages in the uremic rats are polarized towards a proinflammatory phenotype and assist bacterial translocation resulting in microinflammation. However, it is still unclear what kind of mechanism activates intestinal macrophages in uremia.
Methods
Male Sprague-Dawley rats were randomly divided into two groups: sham, uremia. Immunochemistry was used to analyze the expression of macrophage-inducible C-type lectin (Mincle). RT-PCR and western blot were employed to assess the mRNA and protein expression of toll-like receptor 4 (TLR4).
Results
Our RCT study found that the number of Lactobacilli in the intestines of patients with end-stage diabetic nephropathy was significantly higher than that in non-diabetic patients. The plasma levels of endotoxin, CRP, IL-6, and TNF-a in the uremia group were greater than those in the sham group (p＞0.05)(Table 1).Compared with the sham group, the uremia group exhibited macrophages with higher staining intensities for Mincle and higher mRNA and protein expression of TLR4(Figure1-2).
Conclusion
The solution to this scientific problem will not only clarify the molecular mechanism of intestinal bacteria in controlling the activation of intestinal macrophages, but also to clarify the micro-inflammation state of uremia.
Table 1. Body weight, hematocrit, and blood chemistry results
| Group (n = 10) | Body weight (g) | Creatinine (µmol/L) | Urea (mmol/L) | Endotoxin (Eu/mL) | CRP(mg/mL) | IL-6(pg/mL) | TNF-α (pg/mL) |
| Sham | 560.4 ±16.6 | 31.5 ±6.7 | 6.05 ±0.85 | 0.016±0.005 | 2.48 ±0.28 | 18.26 ±3.72 | 20.9 ±0.28 |
| Uremia | 516.6 ±14.5 | 95.7 ±35.6* | 18.10 ±8.50* | 0.033 ±0.009* | 5.2 ±0.77* | 31.07 ±10.06* | 30.4 ± 7.73* |
Data are presented as the mean ±SD. *p < 0.05 vs. the sham group
Funding
- Government Support - Non-U.S.