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Kidney Week

Abstract: PO0631

Study on the Mechanism of Microinflammation with Uremia Caused by Lactobacillus Activation of Intestinal Macrophages by Mincle

Session Information

  • CKD Mechanisms - 2
    October 22, 2020 | Location: On-Demand
    Abstract Time: 10:00 AM - 12:00 PM

Category: CKD (Non-Dialysis)

  • 2103 CKD (Non-Dialysis): Mechanisms

Authors

  • Sun, Lingshuang, First Affiliated Hospital of Medicine School, Xi’an Jiaotong University,, Xi'an, Shaanxi, China
  • Jiang, Hongli, First Affiliated Hospital of Medicine School, Xi’an Jiaotong University,, Xi'an, Shaanxi, China
Background

My previous studies have found that Intestinal macrophages in the uremic rats are polarized towards a proinflammatory phenotype and assist bacterial translocation resulting in microinflammation. However, it is still unclear what kind of mechanism activates intestinal macrophages in uremia.

Methods

Male Sprague-Dawley rats were randomly divided into two groups: sham, uremia. Immunochemistry was used to analyze the expression of macrophage-inducible C-type lectin (Mincle). RT-PCR and western blot were employed to assess the mRNA and protein expression of toll-like receptor 4 (TLR4).

Results

Our RCT study found that the number of Lactobacilli in the intestines of patients with end-stage diabetic nephropathy was significantly higher than that in non-diabetic patients. The plasma levels of endotoxin, CRP, IL-6, and TNF-a in the uremia group were greater than those in the sham group (p>0.05)(Table 1).Compared with the sham group, the uremia group exhibited macrophages with higher staining intensities for Mincle and higher mRNA and protein expression of TLR4(Figure1-2).

Conclusion

The solution to this scientific problem will not only clarify the molecular mechanism of intestinal bacteria in controlling the activation of intestinal macrophages, but also to clarify the micro-inflammation state of uremia.

Table 1. Body weight, hematocrit, and blood chemistry results
Group (n = 10)Body weight (g)Creatinine (µmol/L)Urea (mmol/L)Endotoxin (Eu/mL)CRP(mg/mL)IL-6(pg/mL)TNF-α (pg/mL)
Sham560.4 ±16.631.5 ±6.76.05 ±0.850.016±0.0052.48 ±0.2818.26 ±3.7220.9 ±0.28
Uremia516.6 ±14.595.7 ±35.6*18.10 ±8.50*0.033 ±0.009*5.2 ±0.77*31.07 ±10.06*30.4 ± 7.73*

Data are presented as the mean ±SD. *p < 0.05 vs. the sham group

Funding

  • Government Support - Non-U.S.