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Kidney Week

Abstract: PO2617

Protective Effects of Apelin on Contrast-Induced Nephropathy

Session Information

Category: Acute Kidney Injury

  • 101 AKI: Epidemiology, Risk Factors, and Prevention

Authors

  • Kim, Jae seok, Wonju Severance Christian Hospital, Wonju, Gangwon-do, Korea (the Republic of)
  • Kim, Miryung, Wonju Severance Christian Hospital, Wonju, Gangwon-do, Korea (the Republic of)
  • Shin, Hanwul, Wonju Severance Christian Hospital, Wonju, Gangwon-do, Korea (the Republic of)
  • Lee, Jun Young, Wonju Severance Christian Hospital, Wonju, Gangwon-do, Korea (the Republic of)
  • Yang, Jae Won, Wonju Severance Christian Hospital, Wonju, Gangwon-do, Korea (the Republic of)
  • Eom, Minseob, Wonju Severance Christian Hospital, Wonju, Gangwon-do, Korea (the Republic of)
  • Choi, Seung-Ok, Wonju Severance Christian Hospital, Wonju, Gangwon-do, Korea (the Republic of)
Background

Contrast induced nephropathy (CIN) has no proven preventive measures yet except for saline administration. Apelin is a endogenous ligand to apelin receptor in body, which has physiologic roles such as increasing cardiac output and peripheral vasodilation. This study aims to examine the protective effect of apelin on CIN.

Methods

A total of 22 rats were divided into 4 groups: Control, Apelin, Contrast, and Apelin/Contrast. In order to effectively induce CIN, 50 mg/kg of gentamycin IV was injected daily to all rats from Day-1 to Day-6. On Day-7, the rats were pre-treated as follows; Control and Contrast groups: Saline SQ, Apelin and Apelin/Contrast groups: apelin-13, 300 μg/kg SQ. After 1-hour of pre-treatment, main treatments were followed; Control and Apelin groups: Saline IV, Contrast and Apelin/Contrast groups: Iohexol-350 mg Id/mL, 1.8 g Id/kg IV. We performed serum and 24-hour urine tests on Day-0 and Day-9 which was 48 hours after contrast administration. We collected the kidney with sacrifice on Day-9.

Results

The results showed a significant increase in serum creatinine (Cr) in only Contrast group (p=0.039). In Contrast (p=0.015) and Apelin/Contrast (p=0.007) groups, urinary Cr excretion significantly increased indicating renal tubular injury. The immunohistochemistry for caspase-3 indicated that Contrast group had significant tubular cell damages, while other groups including Apelin/Constrast were less damaged (p=0.014).

Conclusion

We could identify the protective effects of apelin against CIN in the study.