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Kidney Week

Abstract: PO0606

FoxM1 Inhibition Ameliorates Renal Interstitial Fibrosis (RIF) by Decreasing Extracellular Matrix and Epithelial-to-Mesenchymal Transition

Session Information

  • CKD Mechanisms - 1
    October 22, 2020 | Location: On-Demand
    Abstract Time: 10:00 AM - 12:00 PM

Category: CKD (Non-Dialysis)

  • 2103 CKD (Non-Dialysis): Mechanisms

Authors

  • Hui, Wang Yan, Xiangya Hospital, Central South University, Changsha, Hunan, China
  • Zhou, Qiaoling, Xiangya Hospital, Central South University, Changsha, Hunan, China
Background

FoxM1 is a transcriptional regulator involved in tumor development, pulmonary fibrosis, and cardiac fibrosis. However, its role in RIF has yet to be elucidated.

Methods

We established a TGF-β1-stimulated human proximal tubular epithelial cell (HK-2) model in vitro and a unilateral ureteral obstruction (UUO)-induced rat RIF model in vivo. FoxM1 inhibition was achieved by siRNA interference in vitro and by injecting thiostrepton into UUO-induced RIF rats in vivo. The degree of renal damage and fibrosis were determined by histological assessment via hematoxylin and eosin staining. Immunohistochemistry, western blots, and qPCR were used to determine the expression levels of FoxM1, Collagen I, E-cadherin, α-SMA, and Snail1

Results

FoxM1 inhibition could ameliorate RIF and reduce the deposition of Collagen I. H&E staining revealed that renal structural damage, inflammatory cell infiltration, and ECM deposition were significantly attenuated by thiostrepton treatment in the UUO rats. FoxM1 downregulation significantly suppressed EMT, as evidenced by decreased protein and mRNA expression levels of α-SMA and Snail1 and a significant increase in protein and mRNA expression levels of E-cadherin.

Conclusion

FoxM1 inhibition could be a novel therapeutic strategy for the treatment of RIF.

Protein levels in TGF-β1-induced HK-2 cells.FoxM1 downregulation inhibited TGF-β1-induced EMT