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Abstract: PO1713

The Correlation Between Urinary MicroRNA-21 and Renal Parameters in Patients with IgA Nephropathy

Session Information

Category: Glomerular Diseases

  • 1201 Glomerular Diseases: Fibrosis and Extracellular Matrix

Authors

  • Araumi, Akira, Yamagata University Faculty of Medicine, Yamagata, Japan
  • Osaki, Tsukasa, Yamagata University Faculty of Medicine, Yamagata, Japan
  • Ichikawa, Kazunobu, Yamagata University Faculty of Medicine, Yamagata, Japan
  • Watanabe, Masafumi, Yamagata University Faculty of Medicine, Yamagata, Japan
  • Konta, Tsuneo, Yamagata University Faculty of Medicine, Yamagata, Japan
Background

The expression of microRNA-21 (miR-21) in renal tissue is reported to be related to tubulointerstitial fibrosis and renal outcome in IgA nephropathy. In this study, we examined whether the urinary concentration of miR-21 is related to clinicopathological parameters and short-term changes in renal function in patients with IgA nephropathy.

Methods

We extracted and quantified microRNAs in morning spot urine in 88 patients with IgA nephropathy at biopsy and five control subjects, and examined the relationship between clinical and histological parameters, one-year changes in eGFR and urinary miR-21. The concentrations of microRNAs and proteins were corrected to the concentration of urinary creatinine and were log-transformed for simple correlation analysis.

Results

The urinary excretion of miR-21 was detected in all subjects, and the urinary concentration of miR-21 in patients with IgA nephropathy was significantly higher than those in controls. Among 88 patients with IgA nephropathy, urinary miR-21 levels showed a significantly positive correlation with the urinary concentration of total microRNA (r=0.65), total protein (r=0.40), beta2-microglobulin (r=0.62), and N-acetyl-beta-D-glucosaminidase (NAG) (r=0.37), but not with baseline GFR, and urinary red blood cells. In contrast, the urinary miR-21 levels did not show a significant correlation with histological changes, including glomerular proliferation/sclerosis and tubulointerstitial fibrosis. The one-year changes in eGFR after biopsy showed a significant inverse correlation with the urinary concentration of miR-21 (r=-0.31) and total protein (r=-0.37), but not total microRNA, beta2-microglobulin, and NAG. The correlation between urinary miR-21 and one-year eGFR change was similar in the subjects with and without steroid treatment.

Conclusion

In this study, the urinary excretion of miR-21 was associated with clinical parameters and one-year changes in renal function in patients with IgA nephropathy, suggesting that urinary miR-21 might be used as a biomarker of IgA nephropathy.

Funding

  • Government Support - Non-U.S.