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Kidney Week

Abstract: PO0231

Lipidomics Approach to Characterize Lipid Alterations in Human Kidney 2 Cells Treated with Iohexol

Session Information

  • AKI Mechanisms - 3
    October 22, 2020 | Location: On-Demand
    Abstract Time: 10:00 AM - 12:00 PM

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Zhou, Fangfang, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China
  • Chen, Yixin, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China
  • Xin, Luo Lian, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China
  • Lu, Yi, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China
  • Luo, Qun, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, China
Background

In our previous study, we found plasma high density lipoprotein cholesterol and apolipoprotein A-I levels were significant lower in the contrast induced nephropathy (CIN) group, compared to the non-CIN group. The aims of our study was to uncover lipid alterations that may contribute to the development of CIN.

Methods

We investigated lipids of HK-2 cells treated with iohexol (100mg I/ml, 6hrs and 12hrs) , compared with HK-2 cells treated without iohexol (as control) using nontargeted lipidomics based on ultrahigh-performance liquid chromatography-Orbitrap Q-Exactive plus mass spectrometry.

Results

Based on the principles of t-test P-value < 0.05, variable importance at projection (VIP) value >1 and fold change >1.5 or <0.67, differential expression of 134 lipids were identified in both HK-2 cells treated with iohexol for 6hrs and 12hrs, compared with the control HK-2 cells . The top three class to which these lipids belonged were phosphatidylcholine (PC, 42 species), triglyceride(TG, 39 species) and phosphatidylethanolamine (PE, 18 species).

Conclusion

Our results indicate that PC, TG and PE may contribute to the development of CIN, providing the basis for further evaluation of the role of lipids in the mechanism of CIN.