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Abstract: PO2014

Lnc-Gm43360 Regulates TCMK-1 Senescence by the miR-141/Sirt1 Pathway

Session Information

Category: Health Maintenance, Nutrition, and Metabolism

  • 1300 Health Maintenance, Nutrition, and Metabolism

Authors

  • Li, Jie, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China
  • Jiang, Hongli, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China
  • Chen, Lei, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China
Background

Aging is a complex process, which will lead to the gradual decline of physiological functions of all organ systems. The kidney, which is a metabolically active organ, is extremely susceptible to aging, but the mechanism of kidney aging is not clear. Long-chain non-coding RNA (lncRNA) is an non-coding RNA consisting of 200 nucleotides, which play an important role in kidney fibrosis and diabetic nephropathy, but there is no study on kidney senescence.

Methods

Dectection of lnc-Gm43360 expression by qRT-PCR. Transfection with lnc-Gm43360 siRNA and overexpressed plasmid to measure the miR-141 and Sirt1 expression by qRT-PCR, and the p53, p21 and p16 expression by western blot, and SA-β-gal expression. Transfection with miR-141 mimic and inhibitor to measure Sirt1 expression by qRT-PCR, and p53, p21 and p16 expression by western blot, and and SA-β-gal expression.

Results

Lnc-Gm43360 expression in 24-month-mouse lower than 3-month-mouse kidney tissue. The reduction of lnc-Gm43360 expression significantly increases miR-141 expression, decrease Sirt1 expression on both the mRNA and protein level, and induces the SA-β-gal expression. miR-141 mimic and inhibitor decrease and increase Sirt1 expression. lnc-Gm43360 negatively regulates miR-141 expression and positive negatively regulate Sirt1 expression at both the mRNA and protein level. The function of lnc-Gm43360 in regulating Sirt1 expression depends on modulating miR-141 expression.

Conclusion

Lnc-Gm43360 can induce TCMK-1 senescence by miR-141/Sirt1 pathway.