Abstract: PO1418
Urine and Plasma Complement Ba Levels During Flares of Nephritis in Patients with ANCA-Associated Vasculitis
Session Information
- Glomerular Diseases: Immunology and Inflammation in Vasculitis and Lupus Nephritis
November 04, 2021 | Location: On-Demand, Virtual Only
Abstract Time: 10:00 AM - 12:00 PM
Category: Glomerular Diseases
- 1202 Glomerular Diseases: Immunology and Inflammation
Authors
- Almaani, Salem, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States
- Song, Huijuan, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States
- Suthanthira, Meshora, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States
- Toy, Christopher, Colorado State University System, Denver, Colorado, United States
- Fussner, Lynn A., The Ohio State University Wexner Medical Center, Columbus, Ohio, United States
- Meara, Alexa Simon, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States
- Nagaraja, Haikady N., The Ohio State University Wexner Medical Center, Columbus, Ohio, United States
- Khalidi, Nader A., McMaster University, Hamilton, Ontario, Canada
- Pagnoux, Christian, Mount Sinai Hospital, Toronto, Ontario, Canada
- Warrington, Kenneth, Mayo Foundation for Medical Education and Research, Rochester, Minnesota, United States
- Monach, Paul, Brigham and Women's Hospital, Boston, Massachusetts, United States
- Merkel, Peter A., University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, United States
- Rovin, Brad H., The Ohio State University Wexner Medical Center, Columbus, Ohio, United States
- Birmingham, Daniel J., The Ohio State University Wexner Medical Center, Columbus, Ohio, United States
Group or Team Name
- Vasculitis Clinical Research Foundation
Background
The alternative complement pathway has been implicated in the pathogenesis of ANCA-associated vasculitis (AAV), however it is not clear whether activation of complement occurs systemically or in affected organs such as the kidney. This study measured levels of urinary and plasma complement fragment Ba (uBa and pBa respectively) at multiple timepoints in patients with AAV.
Methods
Ba was measured by ELISA in serial samples of urine (uBa) and plasma (pBa) from 20 AAV patients who developed a renal flare, 20 who developed a non-renal flare, and 20 in long-term remission. Changes in Ba levels were modeled using linear mixed effect models.
Results
Cohort characteristics are given in Figure.1. uBa levels increased at renal flare , but did not increase at non-renal flare , and remained stable in long-term remission (Figure 2a). pBa levels were stable over time in all groups (Figure 2b). uBa correlated with renal AAV activity measured as the renal component of the BVAS score (R2= 0.33, p<0.01), but did not correlate with the overall BVAS score during renal flare (R2= 0.13, p=0.12) or non-renal flare (R2= 0.10, p=0.22).
Conclusion
Urine, but not plasma, Ba levels increase at the time of a flare of renal disease in AAV, suggesting intra-renal alternative complement pathway activation. uBa has the potential for use as a surveillance biomarker of renal vasculitis.
Funding
- Other NIH Support