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Kidney Week

Abstract: PO0359

Role and Regulatory Mechanism of Adropin in AKI by Regulating PDK4

Session Information

Category: Acute Kidney Injury

  • 103 AKI: Mechanisms

Authors

  • Huang, Mingcheng, Sun Yat-Sen University, Guangzhou, China
  • Jiang, Shan, Sun Yat-Sen University, Guangzhou, China
  • Wu, Keping, Sun Yat-Sen University, Guangzhou, China
  • Wang, Xiaohua, Sun Yat-Sen University, Guangzhou, China
  • Lei, Yan, Sun Yat-Sen University, Guangzhou, China
  • Tian, Xiuxun, Sun Yat-Sen University, Guangzhou, China
  • Zhang, Lanyue, Sun Yat-Sen University, Guangzhou, China
  • Liu, Yu, Sun Yat-Sen University, Guangzhou, China
  • Cui, Tianjiao, Sun Yat-Sen University, Guangzhou, China
  • Zheng, Zhihua, Sun Yat-Sen University, Guangzhou, China
Background

Oxidative stress and inflammation are the important biological mechanisms of the development of ischemic Acute kidney injury (AKI). Pyruvate Dehydrogenase Kinase 4 (PDK4) is a key enzyme in the process of glucose oxidation, which can inhibit glucose oxidation.Adropin, a secreted protein encoded by Energy Homeostasis Associated (ENHO) gene, is involved in the pathogenesis and pathological process of metabolic and inflammatory diseases and other diseases, can inhibit oxidative stress and inflammation, but its mechanism is unclear in AKI.

Methods

In vitro, HK2 cells were incubated with rotenone to mimicked Ischemia Reperfusion Injury (mIRI) and treated with Adropin, the changes of mitochondrial membrane potential, indicators of autophagy, antioxidant protein, ROS levels and PKD4 were detected. In vivo, we established AKI model with IRI, and Adropin was given intravenously to detect the changes of renal injury indexes, antioxidant protein(SOD2), Adropin and PKD4 in mice.

Results

1. Adropin up-regulated the antioxidant enzyme SOD2 and decreased the ROS level in HK-2 cells with mimicked Ischemia Reperfusion Injury.
2. The expression of PDK4 was significantly up-regulated and SOD2 was down-regulated in AKI mice induced by IRI. With the treatment of Adropin, the expression of SOD2 was up-regulated, and renal injury was alleviated.

Conclusion

Adropin can reduce the production of ROS by down-regulating the expression of PDK4 and up-regulating SOD2, thus alleviating renal injury in AKI.